PKAα/β cat Antibody (62A C2A): sc-81174

PKAα/β cat Antibody (62A C2A) is a mouse monoclonal IgG1 antibody engineered to detect the catalytic subunits alpha and beta of protein kinase A (PKA) in bovine samples through western blotting (WB) and immunofluorescence (IF) assays. Anti-PKAα/β cat antibody (62A C2A) is raised against the cyclic AMP kinase catalytic subunit isolated from bovine cardiac muscle, ensuring precise targeting of PKAα and PKAβ proteins. Protein kinase A is a critical cAMP-dependent enzyme that plays a pivotal role in regulating a wide array of cellular processes, including proliferation, ion transport, metabolism, and gene transcription. Structurally, PKAα/β cat consists of a conserved kinase domain responsible for its catalytic activity and regulatory domains that bind cyclic AMP (cAMP), allowing for tight regulation of its function. Upon binding of cAMP to the regulatory subunits, the active catalytic subunits are released to phosphorylate various substrates. PKAα and PKAβ catalytic subunits interact with several key proteins that facilitate their specific functions within the cell. Notably, A-kinase anchoring proteins (AKAPs) bind to PKAα/β cat, localizing the kinase to specific subcellular compartments and ensuring targeted phosphorylation of substrates. Additionally, PKAα/β cat interacts with transcription factors such as the cAMP response element-binding protein (CREB), phosphorylating it on Serine 133 to regulate gene expression. These interactions are crucial for the precise modulation of signaling pathways that control essential physiological processes. PKAα/β cat monoclonal antibody (62A C2A) enables researchers to investigate the dynamic regulation and functional mechanisms of PKA, including its interactions with AKAPs and CREB, within various physiological and pathological contexts.

The preferred PKAα cat antibody is PKAα cat Antibody (A-2): sc-28315.