PKAα/β cat Antibody (3C1): sc-52153

PKAα/β cat Antibody (3C1) is a mouse monoclonal IgG1 antibody designed to detect the catalytic subunits alpha and beta of protein kinase A (PKA) in both human and bovine samples using western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). PKAα/β cat monoclonal antibody (3C1) is raised against purified PKAα catalytic subunit derived from bovine cardiac muscle, ensuring precise targeting of the PKAα and PKAβ proteins. Protein kinase A is a crucial cAMP-dependent enzyme that exists as a tetrameric holoenzyme composed of two regulatory and two catalytic subunits. Upon binding of the second messenger cyclic AMP (cAMP) to the regulatory subunits, the active catalytic subunits are released, enabling them to phosphorylate various substrates involved in diverse cellular processes such as proliferation, ion transport, metabolism, and gene transcription. The close structural similarity between PKAα cat and PKAβ cat, with 93% amino acid sequence similarity, highlights the ability of PKAα/β cat monoclonal antibody (3C1) to reliably recognize both isoforms, while distinguishing them from the less similar PKAγ cat. Understanding the intricate structure of PKA is essential for elucidating its role in signal transduction pathways, particularly its function in phosphorylating the cAMP response element-binding protein (CREB) on Serine 133, which is pivotal for initiating transcription by linking to the Pol II transcription initiation complex. Anti-PKAα/β cat antibody (3C1) provides researchers with a powerful tool to investigate the dynamic regulation and functional mechanisms of PKA in various physiological and pathological contexts.

The preferred PKAα cat antibody is PKAα cat Antibody (A-2): sc-28315.