PITHD1 Antibody (A-10): sc-515392

PITHD1 Antibody (A-10) is a mouse monoclonal IgG3 kappa light chain antibody that detects PITHD1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PITHD1 (A-10) antibody is available as the non-conjugated form. PITHD1 protein, also known as PITH (C-terminal proteasome-interacting domain of thioredoxin-like) domain containing 1, HT014, TXNL1CL, or C1orf128, is a 211 amino acid nuclear protein characterized by the presence of a single PITH domain. PITHD1 plays a crucial role in megakaryocytic differentiation and enhances the expression of RUNX1, a key transcription factor involved in hematopoiesis. PITHD1 regulates RUNX1 expression through two mechanisms: by increasing the transcriptional activity of its proximal promoter and by enhancing the translational activity of an internal ribosome entry site (IRES) element. Altered expression of PITHD1 has been linked to the development of leukemia, with significant downregulation observed in leukemic samples, highlighting its potential as a biomarker for this disease. PITHD1 is located on chromosome 1 and exhibits conservation across a wide range of species, including chimpanzees, Rhesus monkeys, dogs, cows, mice, rats, chickens, zebrafish, fruit flies, mosquitoes, Caenorhabditis elegans, Schizosaccharomyces pombe, Magnaporthe oryzae, Neurospora crassa, Arabidopsis thaliana, rice, and frogs, underscoring its evolutionary significance.

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PITHD1 Antibody (A-10) References:

1. New insights into the evolution of chromosome 1.  |  Weise, A., et al. 2005. Cytogenet Genome Res. 108: 217-22. PMID: 15545733
2. Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress.  |  Guo, D., et al. 2005. Biochem Biophys Res Commun. 337: 1308-18. PMID: 16236267
3. The DNA sequence and biological annotation of human chromosome 1.  |  Gregory, SG., et al. 2006. Nature. 441: 315-21. PMID: 16710414
4. Ubiquitin-mediated proteolysis of HuR by heat shock.  |  Abdelmohsen, K., et al. 2009. EMBO J. 28: 1271-82. PMID: 19322201
5. Proteomic characterization of the human sperm nucleus.  |  de Mateo, S., et al. 2011. Proteomics. 11: 2714-26. PMID: 21630459
6. Novel function of PITH domain-containing 1 as an activator of internal ribosomal entry site to enhance RUNX1 expression and promote megakaryocyte differentiation.  |  Lu, B., et al. 2015. Cell Mol Life Sci. 72: 821-32. PMID: 25134913

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PITHD1 Antibody (A-10) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PITHD1 Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PITHD1 Antibody (A-10) Specifications

Alternate Product Name: PITHD1

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 13-26

Epitope Target: PITHD1

Epitope Species: human

Product Reactivity/Detection: PITHD1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PITHD1 Antibody (A-10): P: Blocking Peptide