PiT1/2 Antibody (SY-12): sc-101298

PiT1/2 Antibody (SY-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects PiT1/2 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-PiT1/2 antibody (SY-12) is available as a non-conjugated format. PiT1 and PiT2 proteins, members of the SLC20 family, are crucial sodium-dependent phosphate transporters that play a significant role in maintaining phosphate homeostasis within cells. These proteins are predominantly located on the membranes of polarized epithelial cells, particularly in tissues such as bone, kidney, and intestine, where they facilitate the cotransport of sodium and phosphate ions. This function is vital for various physiological processes, including bone mineralization and cellular signaling. Notably, PiT2 also serves as a receptor for the amphotropic murine leukemia virus (A-MuLV), which has implications in gene therapy as A-MuLV can infect a wide range of mammalian cell lines, including human cells. The ability of A-MuLV to utilize PiT1 and PiT2 for entry into cells makes these transporters important targets in the development of retroviral vector systems for gene transfer technologies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PiT1/2 Antibody (SY-12) References:

1. Identification of envelope determinants of feline leukemia virus subgroup B that permit infection and gene transfer to cells expressing human Pit1 or Pit2.  |  Sugai, J., et al. 2001. J Virol. 75: 6841-9. PMID: 11435563
2. Pit2 assemblies at the cell surface are modulated by extracellular inorganic phosphate concentration.  |  Salaün, C., et al. 2002. J Virol. 76: 4304-11. PMID: 11932396
3. Two highly conserved glutamate residues critical for type III sodium-dependent phosphate transport revealed by uncoupling transport function from retroviral receptor function.  |  Bottger, P. and Pedersen, L. 2002. J Biol Chem. 277: 42741-7. PMID: 12205090
4. Expression of PiT1 and PiT2 retroviral receptors and transduction efficiency of tumor cells.  |  Grabarczyk, P., et al. 2002. Acta Biochim Pol. 49: 333-9. PMID: 12362974
5. The central half of Pit2 is not required for its function as a retroviral receptor.  |  Bøttger, P. and Pedersen, L. 2004. J Virol. 78: 9564-7. PMID: 15308749
6. Cell signaling through the protein kinases cAMP-dependent protein kinase, protein kinase Cepsilon, and RAF-1 regulates amphotropic murine leukemia virus envelope protein-induced syncytium formation.  |  Wang, W., et al. 2005. J Biol Chem. 280: 16772-83. PMID: 15741175
7. Hepatic regeneration promotes engraftment of intraportally transplanted islet cells.  |  Sudo, T., et al. 2005. Surgery. 137: 612-9. PMID: 15933627
8. Sodium-phosphate cotransporter in human salivary glands: molecular evidence for the involvement of NPT2b in acinar phosphate secretion and ductal phosphate reabsorption.  |  Homann, V., et al. 2005. Arch Oral Biol. 50: 759-68. PMID: 15970207
9. Expression of the rat renal PiT-2 phosphate transporter.  |  Leung, JC., et al. 2005. Horm Metab Res. 37: 265-9. PMID: 15971147
10. Caveola-dependent endocytic entry of amphotropic murine leukemia virus.  |  Beer, C., et al. 2005. J Virol. 79: 10776-87. PMID: 16051869
11. Slc20a1/Pit1 and Slc20a2/Pit2 are essential for normal skeletal myofiber function and survival.  |  Chande, S., et al. 2020. Sci Rep. 10: 3069. PMID: 32080237
12. An NLR paralog Pit2 generated from tandem duplication of Pit1 fine-tunes Pit1 localization and function.  |  Li, Y., et al. 2024. Nat Commun. 15: 4610. PMID: 38816417

The preferred PiT2 antibody is PiT2 Antibody (B-4): sc-377326.