Phosphate Buffered Saline: 1 L of 20X A 20X buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research.

Phosphate Buffered Saline: 1 L of 20X

Phosphate Buffered Saline: 1 L of 20X is rated 5.0 out of 5 by 1.
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Alternate Names: Phosphate Buffered Saline: 1 L of 20X is also known as 20X PBS.
Application: Phosphate Buffered Saline: 1 L of 20X is a 20X buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

Phosphate Buffered Saline: 1 L of 20X is a concentrated buffer solution ready for dilution to a 1X Phosphate-buffered saline (PBS) working solution commonly used in biological research. Phosphate Buffered Saline is ideal for maintaining a constant pH, and since it is isotonic and non-toxic to cells, it can be used in a wide variety of biological applications. PBS is frequently used as a wash and dilution buffer, and is also used to take a reference spectrum when measuring protein adsorption.
Additional sizes, formats, and concentrations available:
Phosphate Buffered Saline: 1 L of 1X (sc-362182)
Phosphate Buffered Saline: 100 ml of 10X (sc-362297)
Phosphate Buffered Saline: 500 ml of 10X (sc-362298)
Phosphate Buffered Saline: 1 L of 10X (sc-24946)
Phosphate Buffered Saline: 5 L of 20X (sc-362299)
Phosphate Buffered Saline: powder for 100 ml of 10X (sc-362300)
Phosphate Buffered Saline: powder for 500 ml of 10X (sc-362301)
Phosphate Buffered Saline: powder for 1 L of 10X (sc-362302)
Phosphate Buffered Saline: powder for 5 L of 10X (sc-24947)


References

1. Dulbecco, R. and Vogt, M. 1954. J. Exp. Med. 99: 167-182. PMID: 13130792

2. Wilcox, C.S., et al. 1992. Proc. Natl. Acad. Sci. U.S.A. 89: 11993-11997. PMID: 1281548

3. Fernando, P.N., et al. 2001. J Chromatogr A. 920: 155-162. PMID: 11452994

4. Kang, J., et al. 2009. Anal. Biochem. 386: 251-255. PMID: 19150324

5. Andersson, J. and Dahlgren, U. 2010. J Dent Res. PMID: 20811070

6. Sambrook, et al. 1989. Rnai: Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press. New York. Cold Spring Harbor.Vol. 3.

Usage :
• Prior to use, dilute to a 1X solution and filter through a 0.22 µm filter into a sterile flask. Makes 20 L of 1X PBS buffer with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate. The PBS solution pH will be approximately 7.4.



• If buffer is used in cell culture applications, sterilization of the solution may be necessary. Pre-diluted buffer can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle).


• PBS can be stored at room temperature, but non-sterile solutions may warrant refrigeration to prevent bacterial growth over time. Concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.
pH :
When diluted to a 1X concentration, the pH will be ~7.4.
Storage :
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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Phosphate Buffered Saline: 1 L of 20X  Product Citations

See how others have used Phosphate Buffered Saline: 1 L of 20X. Click on the entry to view the PubMed entry .

Citations 1 to 4 of 4 total

PMID: # 28050230  Li, Y. et al. 2016. Genes Cancer. 7: 292-300.

PMID: # 26114658  Zhou, J. et al. 2015. Autophagy. 11: 1259-79.

PMID: # 12631081  Moe, SM. et al. 2003. Kidney international. 63: 1003-11.

PMID: # 29374705  Anticancer Res. 803-809.

Citations 1 to 4 of 4 total

estimated delivery for this product? PC:nw1 9jx

Asked by: Ioanna
Thank you for your question. For accurate availability, shipping and pricing information regarding our chemical products, please contact Customer Service or reach out to your local distributor.
Answered by: Tech Service
Date published: 2019-10-25

Is this PBS without magnesium and calcium ions?

Asked by: El1806
Thank you for your question. Phosphate Buffered Saline: 1 L of 20X does not contain magnesium or calcium ions (only sodium and potassium). For further questions please contact our technical service.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Can you provide a list of components for this material?

Asked by: hawkeye11
Thank you for your question. This chemical, Phosphate Buffered Saline: 1 L of 20X, contains NaCl, KCl, Sodium Phosphate Dibasic Heptahydrate, Potassium Phosphate Dibasic, and Water.
Answered by: Chemical Support 1
Date published: 2017-02-28
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Rated 5 out of 5 by from Various publications cite the use of Phosphate Various publications cite the use of Phosphate Buffered Saline (PBS) as a generalized buffer in numerous applications. -SCBT Publication Review
Date published: 2015-04-22
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