PHF8 Double Nickase Plasmid (h): sc-407306-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PHF8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PHF8 Double Nickase Plasmid (h) and PHF8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PHF8. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PHF8 Double Nickase Plasmid (h) | sc-407306-NIC | 20 µg | $410.00 | |||
PHF8 Double Nickase Plasmid (h2) | sc-407306-NIC-2 | 20 µg | $410.00 |
PHF8 encodes a JmjC domain–containing histone lysine demethylase that preferentially removes repressive marks such as H3K9me2/1 and can also act on H4K20 methylation states, linking chromatin remodeling to transcriptional control. Through its PHD finger–mediated recognition of H3K4 methylation, PHF8 is recruited to active promoters where it modulates RNA polymerase II–dependent gene expression programs. PHF8 participates in epigenetic regulation of cell-cycle progression, neuronal differentiation, and DNA damage-associated chromatin dynamics. Dysregulation or mutation of PHF8 has been associated with neurodevelopmental phenotypes and cancer-relevant transcriptional networks, making it a useful target for studying chromatin-dependent gene regulation.
PHF8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PHF8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PHF8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PHF8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PHF8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.