Phenylmethylsulfonyl Fluoride (PMSF) is an irreversible inhibitor of serine proteases, such as trypsin, chymotrypsin, and thrombin. It also inhibits acetylcholinesterase (AChE) and phospholipase C (PLC). It affords alkenes by reacting with carbonyl compounds and causes sulfonylation of the active-site serine residues. PMSF also inhibits most cysteine proteases like papain. Commonly used in the preparation of cell lysates to inhibit protein digestion. PMSF can substitute for diisopropylfluorophosphate (DFP; sc-203922), and also inhibits metalloproteases and aspartic proteases. It is a widely used fatty acid amid hydrolase (FAAH) inhibitor for pretreatment of brain membrane preparations when testing CB1 receptor activity.
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See how others have used Phenylmethylsulfonyl Fluoride. Click on the entry to view the PubMed entry .
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