Phemx Antibody (FF-37): sc-81997

Phemx Antibody (FF-37) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Phemx protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Phemx antibody (FF-37) is available as a non-conjugated monoclonal isotype antibody. Phemx, also known as pan-hematopoietic expression protein, PHMX, TSPAN32, or TSSC6, is a crucial member of the tetraspanin family, which plays a significant role in transmembrane signal transduction, cell proliferation, differentiation, and motility. Structurally, Phemx is a multi-pass membrane protein characterized by intracellular N- and C-terminal domains, four transmembrane domains, and two extracellular loops, which are essential for cellular signaling pathways. Phemx is ubiquitously expressed from early embryogenesis through adulthood, with predominant expression in hematopoietic tissues, indicating a vital role in hematopoietic cell function. Phemx interacts with the Integrin αIIb/Integrin β3 complex, which is critical for stabilizing arterial thrombi in platelets and regulating "outside-in" signaling, processes that are important for wound healing. The gene encoding Phemx is located in a significant tumor-suppressor gene region associated with Beckwith-Wiedemann syndrome and various cancers, highlighting potential implications in tumor biology and therapeutic strategies.

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Phemx Antibody (FF-37) References:

1. Two novel genes in the center of the 11p15 imprinted domain escape genomic imprinting.  |  Lee, MP., et al. 1999. Hum Mol Genet. 8: 683-90. PMID: 10072438
2. Sequence conservation and variability of imprinting in the Beckwith-Wiedemann syndrome gene cluster in human and mouse.  |  Paulsen, M., et al. 2000. Hum Mol Genet. 9: 1829-41. PMID: 10915772
3. Phemx, a novel mouse gene expressed in hematopoietic cells maps to the imprinted cluster on distal chromosome 7.  |  Nicholson, RH., et al. 2000. Genomics. 68: 13-21. PMID: 10950922
4. A hematopoietic-specific transmembrane protein, Art-1, is possibly regulated by AML1.  |  Harada, Y., et al. 2001. Biochem Biophys Res Commun. 284: 714-22. PMID: 11396961
5. Molecular characterisation of mouse and human TSSC6: evidence that TSSC6 is a genuine member of the tetraspanin superfamily and is expressed specifically in haematopoietic organs.  |  Robb, L., et al. 2001. Biochim Biophys Acta. 1522: 31-41. PMID: 11718897
6. The absence of Tssc6, a member of the tetraspanin superfamily, does not affect lymphoid development but enhances in vitro T-cell proliferative responses.  |  Tarrant, JM., et al. 2002. Mol Cell Biol. 22: 5006-18. PMID: 12077330
7. Impaired 'outside-in' integrin alphaIIbbeta3 signaling and thrombus stability in TSSC6-deficient mice.  |  Goschnick, MW., et al. 2006. Blood. 108: 1911-8. PMID: 16720835