Phalloidin CruzFluor™ 488 Conjugate MW: ~1900

Phalloidin CruzFluor™ 488 Conjugate

Phalloidin CruzFluor 488 Conjugate is rated 4.6 out of 5 by 5.
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Alternate Names: Phalloidin CruzFluor™ 488 Conjugate also known as Phalloidin-iFluor™ 488 Conjugate
Application: Phalloidin CruzFluor™ 488 Conjugate is a fluorescent phalloidin conjugate that selectively binds to F-actins
Molecular Weight: ~1900
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Phalloidin CruzFluor™ 488 Conjugate is a green fluorescent phalloidin conjugate that is an excellent substitute for AlexFluor® 488. Phalloidin is a heptapeptide phallotoxin produced by Amanita phalloides, the death cap mushroom. Phalloidin binds and stabilizes filamentous F-actin, preventing the dissociation of paired filaments and disturbing the natural equilibrium between filamentous (polymeric) and globular (monomeric) actin in the cell, as well as inhibit the ATP hydrolysis activity of F-actin. Actin is used by the cell for mechanical processes such as growth, scaffolding of the cytoskeleton, and locomotion, and disruption of this actin equilibrium destroys cellular functioning. The magnitude by which the actin protein differs from species to species is no more than 20%.
These properties of Phalloidin make Phalloidin CruzFluor™ Conjugates a fantastic tool for labeling, identifying and quantitating F-actins. The Phalloidin CruzFluor™ Conjugates are used at nanomolar concentrations with cell-free experiments, cell culture experiments, formaldehyde-fixed, permeabilized tissue sections and the study of actin networks. Because Phalloidin CruzFluor™ Conjugates are offered in different colors the series is excellent for multicolor imaging applications.

Usage :
Phalloidin CruzFluor™ conjugates can be used alongside antibodies in IF staining of formalin-fixed cells and formalin-fixed, frozen tissue sections. The Phalloidin will stain F-actin.

1. Prepare 1000 X Phalloidin DMSO stock solution: by adding 30 uL of DMSO into the powder form vials.

2. Prepare 1X Phalloidin conjugate working solution: by adding 1 uL of 1000X Phalloidin conjugate DMSO solution to 1 mL of PBS with 1% BSA

Note 1: The unused 1000X DMSO stock solution of Phalloidin conjugate should be aliquoted and stored at -20° C. protected from light.

Note 2: Different cell types might be stained differently. The concentration of Phalloidin conjugate working solution should be prepared accordingly.


3. Stain the cells or tissue:

3.1 Perform formaldehyde fixation. Incubate cells with 3.0-4.0% formaldehyde in PBS at room temperature for 10-30 minutes.

Note 3: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.

3.2 Rinse the fixed cells 2-3 times in PBS.

3.3 Optional: Add 0.1% Triton X-100 in PBS into fixed cells (from Step 3.2) for 3 to 5 minutes to increase permeability. Rinse the cells 2-3 times in PBS.

3.4 Add Phalloidin conjugate working solution (from Step 2) into the fixed cells (from Step 3.2 or 3.3), and stain the cells at room temperature for 20 to 90 minutes.

3.5 Rinse cells gently with PBS 2-3 times to remove excess Phalloidin conjugate before mounting, sealing and imaging under microscope.

Note 4: If antibody staining is desired, perform blocking, primary antibody and secondary antibody incubations separately between Steps 3.3 and 3.4 according to manufacturer′s protocol. Labeling with Phalloidins can be combined with secondary antibody staining, as long as the correct dilutions of each reagent are used in the staining solution.

Note 5: Paraffin-embedded tissues are not recommended for Phalloidin staining, because the use of solvents during the deparaffinization process will disrupt actin.
Formulation :
1000X stock solution in DMSO; 30 µl total
Physical State :
Liquid
Solubility :
Soluble in DMSO, and water (Low).
Storage :
Store at -20° C
Fluoresence :
λex 493 nm, λem 517 nm in DMSO
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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Phalloidin CruzFluor™ 488 ConjugateProduct Citations

See how others have used Phalloidin CruzFluor™ 488 Conjugate. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 16 total

PMID: # 30266073  Yadav, N.|Jaber, FL.|Sharma, Y.|Gupta, P.|Viswanathan, P.|Gupta, S.| et al. 2019. Hum. Gene Ther. 30: 365-377.

PMID: # 30930164  Ferguson, FM.|Doctor, ZM.|Ficarro, SB.|Browne, CM.|Marto, JA.|Johnson, JL.|Yaron, TM.|Cantley, LC.|Kim, ND.|Sim, T.|Berberich, MJ.|Kalocsay, M.|Sorger, PK.|Gray, NS.| et al. 2019. Cell Chem Biol.

PMID: # 29519603  Sović, I.|Jambon, S.|Kraljević Pavelić, S.|Markova-Car, E.|Ilić, N.|Depauw, S.|David-Cordonnier, MH.|Karminski-Zamola, G.| et al. 2018. Bioorg. Med. Chem. 26: 1950-1960.

PMID: # 30417005  Tan, GZ.|Zhou, Y.| et al. 2018. Nanomicro Lett. 10: 73.

PMID: # 28158906  Thorpe, SD.|Gambassi, S.|Thompson, CL.|Chandrakumar, C.|Santucci, A.|Knight, MM.| et al. 2017. J. Cell. Physiol. 232: 2407-2417.

PMID: # 29254196  Lambertini, E.|Penolazzi, L.|Angelozzi, M.|Grassi, F.|Gambari, L.|Lisignoli, G.|De Bonis, P.|Cavallo, M.|Piva, R.| et al. 2017. Oncotarget. 8: 101686-101696.

PMID: # 26466985  Di Rosa, M. et al. 2016. Immunobiology. 221: 347-56.

PMID: # 27251194  2016. Clinical oral investigations.

PMID: # 26026464  Di Rosa, M. et al. 2015. Inflammation. 38: 2082-91.

PMID: # 23658710  Boehlke, C. et al. 2013. PLoS ONE. 8: -.

Citations 1 to 10 of 16 total

Can this sc-363791 work on paraffin bone tissue?

Asked by: DorothyH
Thank you for your question. We have not tested this on paraffin-embedded bone tissue. We recommend reviewing the product citations to see if anyone else has published using it in bone tissue specifically.
Answered by: Technical Support
Date published: 2019-09-11

Hi, I wonder whether it is possible to get a free sample of Phalloidin FITC to try a trial experiment? Thanks. Gowsy .

Asked by: gowsy
Thank you for your question. We do not currently offer sample sizes of our Phalloidin CruzFluor™ 488 Conjugate: sc-363791. However, we do offer a generous product warranty. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the reagent. Santa Cruz Biotechnology does warranty our products for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement product free of charge, or issue a credit or refund if that is not possible.
Answered by: Technical Support
Date published: 2017-02-03

Is the Phalloidin synthetic or extracted from natural sources?

Asked by: Schrödingers cat
Thank you for your question. Our product, Phalloidin CruzFluor™ 488 Conjugate: sc-363791, is from a synthetic source.
Answered by: Technical Support
Date published: 2016-12-13
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Rated 5 out of 5 by from Good quality Good staining of the cytoskeleton in GBM-derived cells. No bleaching was observed.
Date published: 2019-06-07
Rated 3 out of 5 by from Good product The Phalloidin CruzFluor 488 Conjugate worked great with mouse primary neuronal cell culture but not tissue sections. Although, the staining bleach very soon during confocal imaging.
Date published: 2018-10-02
Rated 5 out of 5 by from high quality and cost effective The staining quality is great. The fluorescence lasted for a week.
Date published: 2018-03-18
Rated 5 out of 5 by from Nice fluorescent staining of the cytoskeleton Nice fluorescent staining of the cytoskeleton.
Date published: 2016-08-09
Rated 5 out of 5 by from Phalloidin CruzFluor 405 Conjugate was used Phalloidin CruzFluor 405 Conjugate was used to visualize F-actin expression in formalin fixed HeLa cells. Crisp green fluorescence observed, crisp. -SCBT QC
Date published: 2015-02-17
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