Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PELP1 Double Nickase Plasmid (m): sc-429042-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PELP1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PELP1 Double Nickase Plasmid (m) and PELP1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Pelp1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PELP1 Antibody (E-1): sc-390599
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PELP1 Double Nickase Plasmid (m) | sc-429042-NIC | 20 µg | $410.00 | |||
PELP1 Double Nickase Plasmid (m2) | sc-429042-NIC-2 | 20 µg | $410.00 |
PELP1 (proline-, glutamic acid-, and leucine-rich protein 1) is a nuclear receptor coregulator and scaffolding protein that integrates estrogen receptor and other steroid receptor signaling with chromatin remodeling and transcriptional control in mouse cells. It coordinates interactions with epigenetic modifiers and transcription complexes, influencing cell-cycle progression, DNA damage responses, and ribosomal biogenesis-related programs. PELP1 has been linked to pathways such as ERα/SRC signaling, MAPK and PI3K/AKT crosstalk, and regulation of gene expression programs that shape proliferation and differentiation. Dysregulated PELP1 activity and localization have been associated with altered hormone signaling and oncogenic transcriptional networks in cancer biology models, supporting mechanistic studies of nuclear receptor-driven phenotypes.
PELP1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Pelp1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Pelp1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Pelp1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Pelp1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.