Pegasus Double Nickase Plasmid (h2): sc-406376-NIC-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pegasus Double Nickase Plasmid (h2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Pegasus Double Nickase Plasmid (h2) and Pegasus Double Nickase Plasmid (h22) encode distinct paired gRNA designs targeting IKZF5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Pegasus Antibody (1B6): sc-517137
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pegasus Double Nickase Plasmid (h2) | sc-406376-NIC-2 | 20 µg | $410.00 |
Human IKZF5 encodes the zinc finger transcription factor Pegasus, a member of the Ikaros family that binds DNA to regulate gene expression programs involved in immune cell differentiation and transcriptional network stability. IKZF5 is implicated in controlling chromatin-associated processes and cytokine-responsive signaling outputs that shape lymphocyte activation states and lineage commitment. Dysregulated IKZF5 activity or expression has been associated with immune-related phenotypes and susceptibility to inflammatory and autoimmune disease mechanisms, supporting its relevance as a molecular node in immunogenetics studies. Gene editing of IKZF5 enables functional interrogation of Pegasus-dependent regulatory circuits, mapping of downstream targets by transcriptomic and epigenomic profiling, and generation of engineered cellular models to study pathway perturbations in human immune biology.
Pegasus Double Nickase Plasmid (h2) consists of a matched pair of plasmids engineered for high-specificity editing of the IKZF5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IKZF5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IKZF5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IKZF5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.