SAM Transcription Activation: SAM system utilizing dCas9-VP64, MS2-P65-HSF1, and sgRNA for precise gene transcription activation.
PEDF CRISPR Activation Plasmid (h): sc-400849-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PEDF CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- PEDF CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by PEDF CRISPR Activation Plasmid (h) and PEDF CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the SERPINF1 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PEDF Antibody (D-10): sc-390172
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PEDF CRISPR Activation Plasmid (h) | sc-400849-ACT | 20 µg | $397.00 | |||
PEDF CRISPR Activation Plasmid (h2) | sc-400849-ACT-2 | 20 µg | $397.00 |