Anti-CD31/PECAM-1 Antibody (H-3) recommended for detection of PECAM-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-CD31/PECAM-1 Antibody (H-3): sc-376764

Anti-CD31/PECAM-1 Antibody (H-3) is rated 4.4 out of 5 by 40.
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Datasheets

    • Anti-CD31/PECAM-1 Antibody (H-3) is a mouse monoclonal IgG1 κ CD31/PECAM-1 antibody, cited in 153 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 699-727 at the C-terminus of PECAM-1 of mouse origin
    • Anti-CD31/PECAM-1 Antibody (H-3) is recommended for detection of PECAM-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-CD31/PECAM-1 Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD31/PECAM-1 (H-3): sc-376764.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CD31/PECAM-1 Antibody (H-3) for WB and IHC(P) applications. These reagents are now offered in bundles with CD31/PECAM-1 Antibody (H-3) (see ordering information below).
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What is the suitable antigen retrieval method for this antibody?

Asked by: DiVa03
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext .1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-09-27

Are trial samples for this antibody offered?

Asked by: james001
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Hola, para este anticuerpo es mejor para bloqueo leche, BSA %??, y que porcentaje de tween? y para lavar, PBS o TBS? Hello, for this antibody is better for blocking milk, BSA, %?? and what percentage of tween? and to wash, PBS or TBS?

Asked by: MARIACONSUELO
Thank you for your question. We recommend blocking with 5% milk or 3% BSA with 0.05% Tween-20 for Western blot. We wash with TBS but PBS would work to. If you have additional questions, please contact Technical Support at 1800-457-3801 option 2, by emailing [email protected] or by live chat on our website at www.scbt.com so we may further assist you.
Answered by: Santa Cruz TS
Date published: 2021-07-07

Hi, can i get a testing aliquot of this antibody for western blot? I need to examine PECAM protein level in human endothelial cells and would like to try out the antibody before we purchase a whole vial. 

Asked by: Yitong
Thank you for your question. Please get in touch with our technical service so we can arrange providing a free sample of this antibody.
Answered by: Tech Support Europe
Date published: 2020-07-08

Hi, can PECAM-1 Anticuerpo (H-3) sc-376764 be used with mouse tissues in histochemistry without any background?

Asked by: Fernandog
Thank you for your question. PECAM Antibody (H-3): sc-376764 is suitable for the detection of PECAM in mouse samples. Depending on the sample, antigen demasking may be necessary. You can find our recommended protocol for immunofluorescence, IHC and different antigen demasking methods by following this link: https://www.scbt.com/scbt/de/resources/protocols/immunofluorescence-cell-staining
Answered by: Tech Service
Date published: 2019-05-20

Is PECAM1 antibody (H3) conjugated to Alexa Fluor 594 stable to fixation and the subsequent solvents such as ethanol and xylene? If kept dark will it still fluoresce?

Asked by: Barb
Thank you for your question. Yes, the Alexa Fluor 594 conjugated antibody is a bright, red-fluorescent dye that provides stable imaging for immunofluorescent staining applications. Alexa Fluor 594 dye is pH-insensitive over a wide molar range. We recommend to perform all steps in the dark. Please see our recommended protocol below for details. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2018-12-12

Is this antibody suitable for IHC of frozen sections, specifically frozen rat brain?

Asked by: harding
Thank you for your question. PECAM-1 (H-3): sc-376764 is recommended for use with frozen tissue sections by IHC. PECAM-1 (H-3) has been demonstrated in several publications to stain rat tissue by IHC.
Answered by: Technical Support
Date published: 2018-12-05

How can I get 10 ul sample test?

Asked by: christiana84186
Thank you for your question! We would be happy to send you a sample of this antibody, sc-376764 SAMPLE. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com, and we'd be glad to get this sample to you!
Answered by: Tech Service
Date published: 2021-02-24
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Rated 1 out of 5 by from Did not work on rat brain tissue We tested this antibody at a range of dilutions on frozen rat brain tissue fixed with either methanol or 4% paraformaldehyde and observed no staining.
Date published: 2022-01-20
Rated 5 out of 5 by from Very good antibody We have used this antibody in WB and worked very well at 1:1000.
Date published: 2020-11-30
Rated 4 out of 5 by from A-FABP (B-4) antibody sc-271529 It is a great Ab which I got as a free sample five month ago.
Date published: 2019-12-06
Rated 5 out of 5 by from Excellent specificity Santa Cruz Biotechnology gives us a sample of its well characterized monoclonal PECAM-1 antibody two months ago and we have obtained excellent results
Date published: 2019-02-18
Rated 5 out of 5 by from Great for WB It works very well on HeLa cell extract. Dilution 1:500.
Date published: 2018-10-08
Rated 2 out of 5 by from IHC-p Marquage des cellules endothéliales de souris mais réaction croisée avec d'autres types cellulaires malgré une très forte saturation des coupes de tissus FFPE
Date published: 2018-04-24
Rated 5 out of 5 by from 功能多样 可以用在多种属,多种实验中,这一点最吸引人,虽然效价比Abcam略低,但是保存方便,只是需要调高浓度才能做出结果
Date published: 2018-02-06
Rated 5 out of 5 by from Antibody Works Fine A relative good antibody for IF experiment to detect CD31 from human origin. HUVEC cells are stained with PECAM-1 (H-3). Antibody was diluted as 1:200 and incubated with sample at 4C overnight. Cell membrane shape can be clearly recognized.
Date published: 2018-01-12
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