Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PEA3 Double Nickase Plasmid (h): sc-401704-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PEA3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PEA3 Double Nickase Plasmid (h) and PEA3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ETV4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PEA3 Antibody (G-10): sc-166629
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PEA3 Double Nickase Plasmid (h) | sc-401704-NIC | 20 µg | $410.00 | |||
PEA3 Double Nickase Plasmid (h2) | sc-401704-NIC-2 | 20 µg | $410.00 |
ETV4 encodes the ETS-family transcription factor PEA3, a DNA-binding regulator that integrates MAPK/ERK-driven signals to control gene programs involved in cell proliferation, differentiation, epithelial–mesenchymal transition, and migration. PEA3 modulates transcription downstream of receptor tyrosine kinases and cooperates with AP-1 and other cofactors to regulate targets linked to extracellular matrix remodeling and invasion-associated phenotypes. Dysregulated ETV4 expression or activity is frequently associated with oncogenic signaling states and altered metastatic behavior across multiple tumor contexts, making it a relevant node for studying transcriptional control of tumor progression. In addition, ETV4 participates in developmental and lineage-specification programs, supporting its use in mechanistic studies of cell-state transitions.
PEA3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ETV4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ETV4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ETV4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ETV4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.