PDZ-RhoGEF Antibody (D-9): sc-166740
- PDZ-RhoGEF Antibody (D-9) is a mouse monoclonal IgG1 κ PDZ-RhoGEF antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 1223-1522 mapping at the C-terminus of PDZ-RhoGEF of human origin
- PDZ-RhoGEF Antibody (D-9) is recommended for detection of PDZ-RhoGEF of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PDZ-RhoGEF Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PDZ-RhoGEF Antibody (D-9). This work is in progress.
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PDZ-RhoGEF Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects PDZ-RhoGEF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDZ-RhoGEF (D-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PDZ-RhoGEF protein, also known as Rho guanine nucleotide exchange factor 11 or ARHGEF11, plays a crucial role in cellular signaling by acting as a guanine nucleotide exchange factor that facilitates GDP for GTP exchange on Rho GTPases, particularly Rho A. This exchange is vital for regulating actin cytoskeleton dynamics and cell morphology, which are essential for processes such as cell migration and axon guidance. PDZ-RhoGEF′s unique ability to bind both nucleotide-free and activated Rho A positions PDZ-RhoGEF as a primary regulator of Rho A activity, influencing various downstream signaling pathways. Notably, mutations in PDZ-RhoGEF′s carboxylate-binding loop can disrupt PDZ-RhoGEF function, leading to altered cell morphology and actin organization, likely due to interaction with MAP-1A (MAP1 light chain LC2). PDZ-RhoGEF′s significance in mediating B plexin-induced activation of Rho/Rho kinase signaling further underscores PDZ-RhoGEF′s importance in developmental processes and cellular responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PDZ-RhoGEF Antibody (D-9) References:
- A novel PDZ domain containing guanine nucleotide exchange factor links heterotrimeric G proteins to Rho. | Fukuhara, S., et al. 1999. J Biol Chem. 274: 5868-79. PMID: 10026210
- Rho-specific binding and guanine nucleotide exchange catalysis by KIAA0380, a dbl family member. | Rümenapp, U., et al. 1999. FEBS Lett. 459: 313-8. PMID: 10526156
- Expression, purification, and crystallization of the RGS-like domain from the Rho nucleotide exchange factor, PDZ-RhoGEF, using the surface entropy reduction approach. | Garrard, SM., et al. 2001. Protein Expr Purif. 21: 412-6. PMID: 11281715
- B plexins activate Rho through PDZ-RhoGEF. | Driessens, MH., et al. 2002. FEBS Lett. 529: 168-72. PMID: 12372594
- Characterization of the expression of PDZ-RhoGEF, LARG and G(alpha)12/G(alpha)13 proteins in the murine nervous system. | Kuner, R., et al. 2002. Eur J Neurosci. 16: 2333-41. PMID: 12492428
- Preliminary crystallographic analysis of the complex of the human GTPase RhoA with the DH/PH tandem of PDZ-RhoGEF. | Oleksy, A., et al. 2004. Acta Crystallogr D Biol Crystallogr. 60: 740-2. PMID: 15039571
- Regulation of RGS-RhoGEFs by Galpha12 and Galpha13 proteins. | Tanabe, S., et al. 2004. Methods Enzymol. 390: 285-94. PMID: 15488184
- Interaction of PDZRhoGEF with microtubule-associated protein 1 light chains: link between microtubules, actin cytoskeleton, and neuronal polarity. | Longhurst, DM., et al. 2006. J Biol Chem. 281: 12030-40. PMID: 16478718
- Activated RhoA binds to the pleckstrin homology (PH) domain of PDZ-RhoGEF, a potential site for autoregulation. | Chen, Z., et al. 2010. J Biol Chem. 285: 21070-81. PMID: 20430886
- Regulation of BCR-dependent germinal center B-cell formation by HGAL and insight into its emerging myeloid ortholog, C1ORF150. | Toran, P., et al. 2024. Front Immunol. 15: 1437516. PMID: 39474423
- Plexin-B1 Directly Interacts with PDZ-RhoGEF/LARG to Regulate RhoA and Growth Cone Morphology | . 2002. Neuron. 35: 51-63.
- B plexins activate Rho through PDZ-RhoGEF | . 2002. FEBS Letters. 529: 168-172.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDZ-RhoGEF Antibody (D-9) | sc-166740 | 200 µg/ml | $316.00 | |||
PDZ-RhoGEF Antibody (D-9) AC | sc-166740 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PDZ-RhoGEF Antibody (D-9) HRP | sc-166740 HRP | 200 µg/ml | $316.00 | |||
PDZ-RhoGEF Antibody (D-9) FITC | sc-166740 FITC | 200 µg/ml | $330.00 | |||
PDZ-RhoGEF Antibody (D-9) PE | sc-166740 PE | 200 µg/ml | $343.00 | |||
PDZ-RhoGEF Antibody (D-9) Alexa Fluor® 488 | sc-166740 AF488 | 200 µg/ml | $357.00 | |||
PDZ-RhoGEF Antibody (D-9) Alexa Fluor® 546 | sc-166740 AF546 | 200 µg/ml | $357.00 | |||
PDZ-RhoGEF Antibody (D-9) Alexa Fluor® 594 | sc-166740 AF594 | 200 µg/ml | $357.00 | |||
PDZ-RhoGEF Antibody (D-9) Alexa Fluor® 647 | sc-166740 AF647 | 200 µg/ml | $357.00 | |||
PDZ-RhoGEF Antibody (D-9) Alexa Fluor® 680 | sc-166740 AF680 | 200 µg/ml | $357.00 | |||
PDZ-RhoGEF Antibody (D-9) Alexa Fluor® 790 | sc-166740 AF790 | 200 µg/ml | $357.00 |