PDPK1 Antibody (5): sc-136243

PDPK1 Antibody (5) is a mouse monoclonal IgG1 light chain antibody that detects PDPK1 in mouse, rat, human, and canine samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). PDPK1, also known as 3-phosphoinositide dependent protein kinase 1, is a crucial 556 amino acid protein that is ubiquitously expressed and localizes to the cell membrane, cytoplasm, and nucleus. As a master kinase, PDPK1 plays a vital role in phosphorylating and activating a subgroup of the AGC family of protein kinases, which are essential for mediating signal transduction pathways that control cell proliferation, survival, and growth, particularly in developing pancreatic beta cells. Additionally, PDPK1 is involved in regulating calcium uptake and calcium-activated potassium channels in mast cells, influencing chemotaxis and motility in vascular endothelial cells, maintaining cardiac homeostasis, and supporting thymocyte development. PDPK1′s structure includes a PH domain that is critical for homodimerization, localization, and nuclear import, alongside a protein kinase domain that facilitates enzymatic activity. PDPK1 exists in five alternatively spliced isoforms and is encoded by a gene located on human chromosome 16p13.3, highlighting its complexity and importance in various cellular processes.

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PDPK1 Antibody (5) References:

1. Genetic inactivation of AKT1, AKT2, and PDPK1 in human colorectal cancer cells clarifies their roles in tumor growth regulation.  |  Ericson, K., et al. 2010. Proc Natl Acad Sci U S A. 107: 2598-603. PMID: 20133737
2. A new microdeletion syndrome involving TBC1D24, ATP6V0C, and PDPK1 causes epilepsy, microcephaly, and developmental delay.  |  Mucha, BE., et al. 2019. Genet Med. 21: 1058-1064. PMID: 30245510
3. Vascular endothelial PDPK1 plays a pivotal role in the maintenance of pancreatic beta cell mass and function in adult male mice.  |  Obata, A., et al. 2019. Diabetologia. 62: 1225-1236. PMID: 31055616
4. Interleukin 1β-mediated HOXC10 Overexpression Promotes Hepatocellular Carcinoma Metastasis by Upregulating PDPK1 and VASP.  |  Dang, Y., et al. 2020. Theranostics. 10: 3833-3848. PMID: 32206125
5. POU2F2 regulates glycolytic reprogramming and glioblastoma progression via PDPK1-dependent activation of PI3K/AKT/mTOR pathway.  |  Yang, R., et al. 2021. Cell Death Dis. 12: 433. PMID: 33931589
6. Leukemia inhibitory factor receptor homodimerization mediated by acetylation of extracellular lysine promotes prostate cancer progression through the PDPK1/AKT/GCN5 axis.  |  Ding, Y., et al. 2022. Clin Transl Med. 12: e676. PMID: 35172032
7. Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotection.  |  Hu, YJ., et al. 2023. iScience. 26: 105932. PMID: 36698722
8. circ_0000376 knockdown suppresses non-small cell lung cancer cell tumor properties by the miR-545-3p/PDPK1 pathway.  |  Sun, C., et al. 2023. Open Med (Wars). 18: 20230641. PMID: 36820067
9. Triple targeting of RSK, AKT, and S6K as pivotal downstream effectors of PDPK1 by TAS0612 in B-cell lymphomas.  |  Katsuragawa-Taminishi, Y., et al. 2023. Cancer Sci. 114: 4691-4705. PMID: 37840379
10. Disruption of Autophagic Flux and Treatment with the PDPK1 Inhibitor GSK2334470 Synergistically Inhibit Renal Cell Carcinoma Pathogenesis.  |  Zhou, W., et al. 2024. J Cancer. 15: 1429-1441. PMID: 38356720

The preferred PDPK1 antibody is PDPK1 Antibody (E-3): sc-17765.