PDILT Antibody (H-5): sc-393529

PDILT Antibody (H-5) is a mouse monoclonal IgG3 kappa light chain antibody that detects PDILT protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PDILT antibody (H-5) is available as a non-conjugated format. PDILT protein, also known as protein disulfide-isomerase-like protein of the testis or PDIA7, is a 584 amino acid protein localized in the endoplasmic reticulum, where PDILT plays a crucial role as a redox-inactive chaperone during spermatogenesis. PDILT protein is specifically expressed in the testis and exists as a homodimer, undergoing post-translational N-glycosylation, which is essential for stability and function. PDILT protein interacts with other proteins such as Calmegin and Ero1-L, which are involved in proper folding and maturation of proteins within the testicular environment. PDILT gene is located on human chromosome 16p12.3, a region that harbors over 900 genes and is implicated in various genetic disorders, including giant axonal neuropathy and Rubinstein-Taybi syndrome. Understanding PDILT interactions and functions is vital for elucidating mechanisms of spermatogenesis and potential implications in male fertility.

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PDILT Antibody (H-5) References:

1. The gene encoding gigaxonin, a new member of the cytoskeletal BTB/kelch repeat family, is mutated in giant axonal neuropathy.  |  Bomont, P., et al. 2000. Nat Genet. 26: 370-4. PMID: 11062483
2. Giant axonal neuropathy (GAN): case report and two novel mutations in the gigaxonin gene.  |  Kuhlenbäumer, G., et al. 2002. Neurology. 58: 1273-6. PMID: 11971098
3. Genetics of inflammatory bowel disease: progress and prospects.  |  Mathew, CG. and Lewis, CM. 2004. Hum Mol Genet. 13 Spec No 1: R161-8. PMID: 14764625
4. PDILT, a divergent testis-specific protein disulfide isomerase with a non-classical SXXC motif that engages in disulfide-dependent interactions in the endoplasmic reticulum.  |  van Lith, M., et al. 2005. J Biol Chem. 280: 1376-83. PMID: 15475357
5. Advances in the genetics of inflammatory bowel disease.  |  Cho, JH. 2004. Curr Gastroenterol Rep. 6: 467-73. PMID: 15527676
6. A developmentally regulated chaperone complex for the endoplasmic reticulum of male haploid germ cells.  |  van Lith, M., et al. 2007. Mol Biol Cell. 18: 2795-804. PMID: 17507649
7. Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility [corrected].  |  Tokuhiro, K., et al. 2012. Proc Natl Acad Sci U S A. 109: 3850-5. PMID: 22357757
8. Structure of the substrate-binding b' domain of the Protein Disulfide Isomerase-Like protein of the Testis.  |  Bastos-Aristizabal, S., et al. 2014. Sci Rep. 4: 4464. PMID: 24662985
9. Crystal and solution structures of human protein-disulfide isomerase-like protein of the testis (PDILT) provide insight into its chaperone activity.  |  Li, H., et al. 2018. J Biol Chem. 293: 1192-1202. PMID: 29203529
10. Genome-Wide Association Study of CKD Progression.  |  Robinson-Cohen, C., et al. 2023. J Am Soc Nephrol. 34: 1547-1559. PMID: 37261792
11. The Rubinstein-Taybi syndrome: occurrence in two sets of identical twins.  |  Baraitser, M. and Preece, MA. 1983. Clin Genet. 23: 318-20. PMID: 6682729
12. Rubinstein-Taybi syndrome caused by submicroscopic deletions within 16p13.3.  |  Breuning, MH., et al. 1993. Am J Hum Genet. 52: 249-54. PMID: 8430691

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PDILT Antibody (H-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PDILT Antibody (H-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PDILT Antibody (H-5) Specifications

Alternate Product Name: PDILT

Product Clone Name: H-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 449-474

Epitope Target: PDILT

Epitope Species: mouse

Product Reactivity/Detection: PDILT

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PDILT Antibody (H-5): P: Blocking Peptide