PDE9A Antibody (D-7): sc-376271
- PDE9A Antibody (D-7) is a mouse monoclonal IgG1 κ PDE9A antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 225-263 within an internal region of PDE9A of human origin
- PDE9A Antibody (D-7) is recommended for detection of PDE9A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine and porcine
- Anti-PDE9A Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PDE9A Antibody (D-7). This work is in progress.
PDE9A Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PDE9A of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-PDE9A antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Phosphodiesterase 9A (PDE9A) is a 593 amino acid protein that plays a crucial role in signal transduction by regulating intracellular concentration of cyclic nucleotides, particularly exhibiting a high affinity for cyclic guanosine monophosphate (cGMP). This regulation influences various physiological processes, including smooth muscle relaxation, neuronal signaling, and cardiac function. PDE9A is expressed in a range of tissues, with highest levels found in brain, kidney, spleen, colon, heart, and testis, while notably absent in blood. PDE9A consists of an N-terminal regulatory domain and a C-terminal catalytic domain, which contains two potential divalent metal ion binding sites, underscoring enzymatic activity. PDE9A has been implicated in affective bipolar disorder, highlighting significance in neuropsychiatric conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PDE9A Antibody (D-7) References:
- Identification and distribution of different mRNA variants produced by differential splicing in the human phosphodiesterase 9A gene. | Rentero, C., et al. 2003. Biochem Biophys Res Commun. 301: 686-92. PMID: 12565835
- Identification and characterization of a new human type 9 cGMP-specific phosphodiesterase splice variant (PDE9A5). Differential tissue distribution and subcellular localization of PDE9A variants. | Wang, P., et al. 2003. Gene. 314: 15-27. PMID: 14527714
- Crystal structure of phosphodiesterase 9 shows orientation variation of inhibitor 3-isobutyl-1-methylxanthine binding. | Huai, Q., et al. 2004. Proc Natl Acad Sci U S A. 101: 9624-9. PMID: 15210993
- Identification and characterization of a novel family of cyclic nucleotide phosphodiesterases. | Soderling, SH., et al. 1998. J Biol Chem. 273: 15553-8. PMID: 9624145
- Isolation and characterization of PDE9A, a novel human cGMP-specific phosphodiesterase. | Fisher, DA., et al. 1998. J Biol Chem. 273: 15559-64. PMID: 9624146
- Identification and characterization of a novel cyclic nucleotide phosphodiesterase gene (PDE9A) that maps to 21q22.3: alternative splicing of mRNA transcripts, genomic structure and sequence. | Guipponi, M., et al. 1998. Hum Genet. 103: 386-92. PMID: 9856478
- Squamous cell carcinoma of the thymus. An analysis of eight cases. | Shimosato, Y., et al. 1977. Am J Surg Pathol. 1: 109-21. PMID: 602973
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDE9A Antibody (D-7) | sc-376271 | 200 µg/ml | $322.00 | |||
PDE9A Antibody (D-7) AC | sc-376271 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PDE9A Antibody (D-7) HRP | sc-376271 HRP | 200 µg/ml | $322.00 | |||
PDE9A Antibody (D-7) FITC | sc-376271 FITC | 200 µg/ml | $336.00 | |||
PDE9A Antibody (D-7) PE | sc-376271 PE | 200 µg/ml | $349.00 | |||
PDE9A Antibody (D-7) Alexa Fluor® 488 | sc-376271 AF488 | 200 µg/ml | $364.00 | |||
PDE9A Antibody (D-7) Alexa Fluor® 546 | sc-376271 AF546 | 200 µg/ml | $364.00 | |||
PDE9A Antibody (D-7) Alexa Fluor® 594 | sc-376271 AF594 | 200 µg/ml | $364.00 | |||
PDE9A Antibody (D-7) Alexa Fluor® 647 | sc-376271 AF647 | 200 µg/ml | $364.00 | |||
PDE9A Antibody (D-7) Alexa Fluor® 680 | sc-376271 AF680 | 200 µg/ml | $364.00 | |||
PDE9A Antibody (D-7) Alexa Fluor® 790 | sc-376271 AF790 | 200 µg/ml | $364.00 | |||
PDE9A (D-7) Neutralizing Peptide | sc-376271 P | 100 µg/0.5 ml | $69.00 |