Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PDE5A Double Nickase Plasmid (m): sc-433880-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PDE5A Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PDE5A Double Nickase Plasmid (m) and PDE5A Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Pde5a. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PDE5A Antibody (D-3): sc-398747
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDE5A Double Nickase Plasmid (m) | sc-433880-NIC | 20 µg | $410.00 | |||
PDE5A Double Nickase Plasmid (m2) | sc-433880-NIC-2 | 20 µg | $410.00 |
Mouse Pde5a encodes phosphodiesterase 5A (PDE5A), a cGMP-specific phosphodiesterase that terminates nitric oxide–cGMP signaling by hydrolyzing cGMP to GMP. By controlling intracellular cGMP levels, PDE5A regulates protein kinase G (PKG) activity and downstream processes including smooth muscle tone, vascular reactivity, platelet function, and cardiomyocyte signaling. PDE5A activity integrates with natriuretic peptide and soluble guanylyl cyclase pathways, shaping cyclic nucleotide cross-talk that influences cellular homeostasis. Altered PDE5A expression or cGMP pathway imbalance has been investigated in models of cardiovascular remodeling, pulmonary vascular dysfunction, and neurovascular regulation.
PDE5A Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Pde5a locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Pde5a. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Pde5a function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Pde5a-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.