PCP-4 Antibody (1E3): sc-293258

PCP-4 Antibody (1E3) is a mouse monoclonal IgG2a kappa light chain antibody that detects PCP-4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-PCP-4 antibody (1E3) is available as the non-conjugated format. PCP-4, also known as Purkinje cell protein 4 or PEP-19, is a calmodulin (CaM) regulatory protein predominantly expressed in neuronal cells, where PCP-4 plays a crucial role in calcium signaling. PCP-4′s unique structure includes an IQ motif that facilitates calcium-dependent interactions with CaM, influencing the dynamics of calcium binding and release from the C-terminal domain of CaM. This interaction is vital for various cellular processes, including synaptic plasticity and neurotransmitter release, which are essential for proper neuronal function. Importantly, the IQ motif contains a serine residue that is subject to phosphorylation by all isoforms of protein kinase C (PKC), a modification that can significantly alter PCP-4′s regulatory capabilities. Dysregulation of PCP-4 has been linked to several pathologies, including uterine leiomyomas and neurodegenerative diseases such as Alzheimer′s and Huntington′s disease, while PCP-4 overexpression is associated with cerebellar hypoplasia, a characteristic feature of Down syndrome.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PCP-4 Antibody (1E3) References:

1. Expression of PEP-19 inhibits apoptosis in PC12 cells.  |  Erhardt, JA., et al. 2000. Neuroreport. 11: 3719-23. PMID: 11117479
2. Small proteins that modulate calmodulin-dependent signal transduction: effects of PEP-19, neuromodulin, and neurogranin on enzyme activation and cellular homeostasis.  |  Slemmon, JR., et al. 2000. Mol Neurobiol. 22: 99-113. PMID: 11414283
3. A new role for IQ motif proteins in regulating calmodulin function.  |  Putkey, JA., et al. 2003. J Biol Chem. 278: 49667-70. PMID: 14551202
4. PEP-19 overexpression in human uterine leiomyoma.  |  Kanamori, T., et al. 2003. Mol Hum Reprod. 9: 709-17. PMID: 14561813
5. Decreased striatal levels of PEP-19 following MPTP lesion in the mouse.  |  Sköld, K., et al. 2006. J Proteome Res. 5: 262-9. PMID: 16457591
6. Genomics, morphogenesis and biophysics: triangulation of Purkinje cell development.  |  Simons, MJ. and Pellionisz, AJ. 2006. Cerebellum. 5: 27-35. PMID: 16527761
7. The influence of phosphorylation on the activity and structure of the neuronal IQ motif protein, PEP-19.  |  Dickerson, JB., et al. 2006. Brain Res. 1092: 16-27. PMID: 16740252
8. Cloning of a rat-specific long PCP4/PEP19 isoform.  |  Sala, A., et al. 2007. Int J Mol Med. 19: 501-9. PMID: 17273800
9. Expression of Pcp4 gene during osteogenic differentiation of bone marrow mesenchymal stem cells in vitro.  |  Xiao, J., et al. 2008. Mol Cell Biochem. 309: 143-50. PMID: 18008138
10. Purkinje cell protein 4 positively regulates neurite outgrowth and neurotransmitter release.  |  Harashima, S., et al. 2011. J Neurosci Res. 89: 1519-30. PMID: 21671256
11. Purkinje Cell Protein 4 Expression Is Associated With DNA Methylation Status in Aldosterone-Producing Adenoma.  |  Kobuke, K., et al. 2018. J Clin Endocrinol Metab. 103: 965-971. PMID: 29294065
12. Circ_0019693 promotes osteogenic differentiation of bone marrow mesenchymal stem cell and enhances osteogenesis-coupled angiogenesis via regulating microRNA-942-5p-targeted purkinje cell protein 4 in the development of osteoporosis.  |  He, W., et al. 2022. Bioengineered. 13: 2181-2193. PMID: 35030971