PCLN-1 Antibody (7A2) recommended for detection of PCLN-1 of mouse, rat and human origin by IF and ELISA

PCLN-1 Antibody (7A2): sc-130561

PCLN-1 Antibody (7A2) is rated 3.0 out of 5 by 1.
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Datasheets

    • Anti-PCLN-1 Antibody (7A2) is a mouse monoclonal IgM (kappa light chain) PCLN-1 antibody provided at 200 µg/ml
    • raised against a peptide sequence corresponding to PCLN-1 of human origin
    • recommended for detection of PCLN-1 of mouse, rat and human origin by IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PCLN-1 (7A2): sc-130561.

What is the recommended fixation method for immunofluorescence staining with PCLN-1 (7A2): sc-130561 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 3 out of 5 by from Produced satisfactory immunofluorescence Produced satisfactory immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2013-05-06
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