Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PB1 Double Nickase Plasmid (m): sc-426270-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PB1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PB1 Double Nickase Plasmid (m) and PB1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Pbrm1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PB1 Antibody (D-8): sc-390095
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PB1 Double Nickase Plasmid (m) | sc-426270-NIC | 20 µg | $410.00 | |||
PB1 Double Nickase Plasmid (m2) | sc-426270-NIC-2 | 20 µg | $410.00 |
Mouse Pbrm1 encodes PB1 (also known as PBRM1/BAF180), a signature subunit of the PBAF SWI/SNF chromatin remodeling complex that recognizes acetylated histones via multiple bromodomains and helps position nucleosomes to regulate transcription. PB1 influences enhancer–promoter communication, DNA damage responses, and lineage-specific differentiation programs by coordinating chromatin accessibility with transcription factor binding. Loss or disruption of Pbrm1 perturbs epigenetic control of cell-cycle and stress-response networks and is frequently studied in the context of tumor suppressor biology, genome stability, and inflammatory signaling. In mouse models, Pbrm1 is widely used to interrogate how chromatin remodelers shape immune microenvironments, metabolic adaptation, and developmental phenotypes.
PB1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Pbrm1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Pbrm1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Pbrm1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Pbrm1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.