Pax-8 Lentiviral Activation Particles (h): sc-401757-LAC

PAX8 encodes Pax-8, a paired box transcription factor required for lineage specification and differentiation in thyroid follicular cells, renal epithelium, and Müllerian-derived tissues. Pax-8 regulates gene programs controlling epithelial identity, organogenesis, and hormone biosynthesis, including transcriptional networks governing thyroid-specific genes and developmental signaling pathways. Altered PAX8 expression or fusion events are associated with tumorigenesis in thyroid and renal contexts, while germline disruption has been linked to congenital hypothyroidism and urogenital developmental defects. In cell models, PAX8 activity influences chromatin states and transcriptional circuitry that shape proliferation, differentiation, and metabolic outputs.

Pax-8 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient PAX8 upregulation across a broader range of human cell types.

Pax-8 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the PAX8 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous Pax-8 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native PAX8 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.