Paraformaldehyde solution 4% in PBS CAS: 30525-89-4

Paraformaldehyde solution 4% in PBS (CAS 30525-89-4)

Paraformaldehyde solution 4% in PBS | CAS 30525-89-4 is rated 4.9 out of 5 by 16.
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Alternate Names: Paraformaldehyde solution 4% in PBS also known as 4% Formaldehyde in PBS, Polyoxymethylene
Application: Paraformaldehyde solution 4% in PBS is commonly used to preserve tissues for routine histology and is supplied in 1X PBS
CAS Number: 30525-89-4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

Paraformaldehyde solution 4% in PBS is commonly used to preserve tissues for routine histology in many labs. Paraformaldehyde solution 4% in PBS solution should be clear, colorless, with no precipitate and the pH 7.0 - 7.6 (25°C). Paraformaldehyde solution 4% is supplied as a 4% Paraformaldehyde solution in 1X PBS. Paraformaldehyde solution 4% in PBS can be used to recognize and stabilize the expression of intracellular antigen.


References

1. Kim, TH. et al. 2016. Scientific reports. 6: 26687. PMID: 27220918

2. Chang, CY. et al. 2016. Nature neuroscience. 19: 111-6. PMID: 26642092

3. Zhou, H. et al. 2015. Stem cell reviews. 11: 652-65. PMID: 25951995

4. Paull, D. et al. 2015. Nature methods. 12: 885-92. PMID: 26237226

Usage :
We recommend solutions are used for no longer than 3 months from when they are made. Aliquot and freeze at -20°C for long-term storage.
Protect from light.
Formulation :
Contains methanol as a stabilizer.
Physical State :
Liquid
Solubility :
Soluble in water
pH :
(25°C): 7.0 - 7.6
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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Paraformaldehyde solution 4% in PBS (CAS 30525-89-4)  Product Citations

See how others have used Paraformaldehyde solution 4% in PBS (CAS 30525-89-4). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 246 total

PMID: # 29523225  Kou, N.|Liu, S.|Li, X.|Li, W.|Zhong, W.|Gui, L.|Chai, S.|Ren, X.|Na, R.|Zeng, T.|Liu, H.| et al. 2019. Oncol. Res. 27: 173-182.

PMID: # 30335546  Chauhan, AS.|Liu, X.|Jing, J.|Lee, H.|Yadav, RK.|Liu, J.|Zhou, Y.|Gan, B.| et al. 2019. FASEB J. 33: 2957-2970.

PMID: # 30917096  2019. Nanomedicine (Lond). 14: 969-987.

PMID: # 31235253  Guo, Y.|Gilbert-Honick, J.|Somers, SM.|Mao, HQ.|Grayson, WL.| et al. 2019. Biochem. Biophys. Res. Commun. 516: 558-564.

PMID: # 31487266  2019. JCI Insight.

PMID: # 31546645  2019. Int J Mol Sci. 20:

PMID: # 31278844  Manousiouthakis, E.|Chen, Y.|Cairns, DM.|Pollard, R.|Gerlovin, K.|Dente, MJ.|Razavi, Y.|Kaplan, DL.| et al. 2019. J Tissue Eng Regen Med.

PMID: # 31455837  2019. Cancer Gene Ther.

PMID: # 31426445  2019. Cancers (Basel). 11:

PMID: # 30867827  Vila, OF.|Uzel, SGM.|Ma, SP.|Williams, D.|Pak, J.|Kamm, RD.|Vunjak-Novakovic, G.| et al. 2019. Theranostics. 9: 1232-1246.

Citations 1 to 10 of 246 total

does this product contain Methanol?

Asked by: Anonymous
Thank you for your question. sc-281692, is a Paraformaldehyde solution 4% in PBS that contains 2.5% methanol.
Answered by: Tech Support Europe
Date published: 2020-06-25

My PFA solution has been kept at 4 C for 7 months, can it still be used for fixing bacterial cells? 

Asked by: Delaney
Thank you for your question. Yes, the recommended storage temperature for this Paraformaldehyde solution 4% in PBS is 4˚C, so it should be fine.
Answered by: Tech Service
Date published: 2020-01-28

Per quanto tempo la soluzione di PFA al 4% si può tenere in frigo a +4°C se non è stata ancora aperta? quando è da considerare scaduta? invece una volta aperta come consigliate di conservarla? per quanto tempo si può tenere aperta a +4°C?

Asked by: Giorgia
Thank you for your question. We recommend solutions are used for no longer than 3 months at 4˚C from when they are made. Aliquot and freeze at -20°C for long-term storage.
Answered by: Tech Service
Date published: 2020-01-23

what is the expiration of the PFA solution if kept at 4 C.

Asked by: Uriw
Thank you for your question. We provide a warranty of one year on the viability of our products given proper storage conditions. To find out the lot-specific expiry date please contact our technical service or your local distributor for expiration information for the specific lot number of your purchased product.
Answered by: Tech Service
Date published: 2019-08-20

Is it suitable for cell fixation during intracellular staining for flow cytometry?

Asked by: Shani
Thank you for your question. We recommend using our FCM Fixation Buffer: sc-3622 for fixing an permeabilizing cells for intracellular staining.
Answered by: Technical Support
Date published: 2019-08-17

Is this product in stock and ready to be shipped out once it is ordered?

Asked by: Curious123
Thank you for your question. Please contact our Customer Care Department for current product availability.
Answered by: Technical Support
Date published: 2019-07-23

What percent methanol does this product contain? Is this product suitable for fixation for downstream detection of endogenous EGFP-tagged protein?

Asked by: Cindy Ohio
Thank you for your question. This product, sc-281692, is a Paraformaldehyde solution 4% in PBS that contains 2.5% methanol. It is used for routine histology fixation, which includes immunofluorescence applications. We can not speak for a specific tagged sample as it is necessary to optimize fixation conditions for every sample, but would expect it to be perfectly fine for that use.
Answered by: Tech Service
Date published: 2019-07-08

would need 5liters for june 6, is this stock ? please confirm price.

Asked by: cobra ben
Thank you for your question. Please contact our Customer Care Department for current product availability, and to request a formal quotation. Current pricing is available on our website.
Answered by: Technical Support
Date published: 2019-01-26
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Rated 5 out of 5 by from Useful Product! I brought this item for our research team member, I personally do not use this.
Date published: 2020-10-05
Rated 5 out of 5 by from Great product It's been the second time of purchasing this product. It works well and save time of preparation.
Date published: 2020-04-28
Rated 5 out of 5 by from great features! My lab has been ordering this and we are very satisfied with it
Date published: 2019-07-02
Rated 5 out of 5 by from Good PFA solution We bought this reagent one month ago. High quality and easy to store and use!
Date published: 2018-04-11
Rated 4 out of 5 by from works for fixation of cells It works for fixation of human cells in culture for cell imaging experiments.
Date published: 2018-02-20
Rated 5 out of 5 by from 4% Paraformaldehyde solution works THe delivery was on time. And the solution worked well. Saved a lot of time to prepare for it.
Date published: 2017-07-05
Rated 5 out of 5 by from Great!!! Ready to use. You do not need to dilute. Nice tissue fixation. Works very well for us.
Date published: 2016-12-30
Rated 5 out of 5 by from Specific and Sensitive Wonderful results using this antibody for IP/WB and IF with human samples.
Date published: 2016-10-28
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