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Paraformaldehyde solution 4% in PBS (CAS 30525-89-4)

Name: Paraformaldehyde solution 4% in PBS | CAS 30525-89-4
Brand: SCBT
SKU: sc-281692
Price: 63.0 USD
Availability: InStock

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Alternate Names:

Paraformaldehyde solution 4% in PBS also known as 4% Formaldehyde in PBS, Polyoxymethylene

Application:

Paraformaldehyde solution 4% in PBS is commonly used to preserve tissues for routine histology and is supplied in 1X PBS

CAS Number:

30525-89-4

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

* Refer to Certificate of Analysis for lot specific data.

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Description

Technical Information

Safety Information

SDS & Certificate of Analysis

Paraformaldehyde solution 4% in PBS is used as a fixative in cell and tissue culture experiments. It functions by cross-linking proteins and nucleic acids, thereby preserving the structural integrity of cells and tissues. This solution works by forming methylene bridges between amino groups of proteins, leading to their stabilization and preventing degradation. Paraformaldehyde solution 4% in PBS aids in the visualization of cellular components and biomolecules by enabling the retention of their spatial organization and morphology. Its mechanism of action involves penetrating the cell membrane and reacting with intracellular molecules to create stable complexes. Paraformaldehyde solution 4% in PBS is particularly useful for immunofluorescence and immunohistochemistry experiments, as it allows for the detection and localization of specific antigens within cells and tissues.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Paraformaldehyde solution 4% in PBS (CAS 30525-89-4) References

Decalcification by ascorbic acid for immuno- and affinohistochemical techniques on the inner ear. | Merchán-Pérez, A., et al. 1999. Histochem Cell Biol. 112: 125-30. PMID: 10460465
Novel 'phage display antibodies identify distinct heparan sulfate domains in developing mammalian lung. | Thompson, SM., et al. 2007. Pediatr Surg Int. 23: 411-7. PMID: 17216534
A simple method for combined fluorescence in situ hybridization and immunocytochemistry. | Moon, IS., et al. 2007. Mol Cells. 24: 76-82. PMID: 17846501
Structural interactions between kisspeptin and GnRH neurons in the mediobasal hypothalamus of the male rhesus monkey (Macaca mulatta) as revealed by double immunofluorescence and confocal microscopy. | Ramaswamy, S., et al. 2008. Endocrinology. 149: 4387-95. PMID: 18511511
The distribution of the prostaglandin E receptor type 2 (EP2) in the detrusor of the guinea pig. | Rahnama'i, MS., et al. 2012. Prostaglandins Other Lipid Mediat. 99: 107-15. PMID: 22960431
Subretinal injection of amyloid-β peptide accelerates RPE cell senescence and retinal degeneration. | Liu, C., et al. 2015. Int J Mol Med. 35: 169-76. PMID: 25385658
Rapid and Efficient Generation of Transgene-Free iPSC from a Small Volume of Cryopreserved Blood. | Zhou, H., et al. 2015. Stem Cell Rev Rep. 11: 652-65. PMID: 25951995
Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells. | Paull, D., et al. 2015. Nat Methods. 12: 885-92. PMID: 26237226
Brief optogenetic inhibition of dopamine neurons mimics endogenous negative reward prediction errors. | Chang, CY., et al. 2016. Nat Neurosci. 19: 111-6. PMID: 26642092
Bio-inspired Hybrid Carbon Nanotube Muscles. | Kim, TH., et al. 2016. Sci Rep. 6: 26687. PMID: 27220918
Experimental data-sets on the histopathological and immunohistological assessment of the Interrenal gland (adrenal homolog) of Japanese medaka (Oryzias latipes) fish exposed to graphene oxide. | Dasmahapatra, AK. and Tchounwou, PB. 2022. Data Brief. 45: 108693. PMID: 36426008
A fixative suitable for in situ hybridization histochemistry. | Uehara, F., et al. 1993. J Histochem Cytochem. 41: 947-53. PMID: 8315285
Flow cytometric detection of EBV (EBER snRNA) using peptide nucleic acid probes. | Just, T., et al. 1998. J Virol Methods. 73: 163-74. PMID: 9766887

Ordering Information

Product Name	Catalog #	UNIT	Price	Qty		FAVORITES
Paraformaldehyde solution 4% in PBS, 1 L	sc-281692	1 L	$63.00

What is the purity of the 4% PFA in PBS?

Asked by: Sarah

Thank you for your question. Purity of this product is not determined. Please contact our technical service team for a CoA of the latest batch of this item or refer to the sample CoA available from the product page right here.

Answered by: Tech Support Europe

Date published: 2023-11-08

Does the 4% solution release formaldehyde gas? or is the product off-gassed prior to shipping?

Asked by: SueFl234

Thank you for your question. This chemical does not release formaldehyde gas. https://datasheets.scbt.com/sds/aghs/en/sc-281692.pdf

Answered by: BlakeJ

Date published: 2023-05-31

Our 4% PFA was received at an ambient temperature. How long can 4% PFA be stored at ambient room temperature?

Asked by: CRH1

Thank you for your question. This product is stable at ambient temperature for the short term but should be kept at +4 °C for long term storage.

Answered by: Tech Support Europe

Date published: 2022-08-03

does this product contain Methanol?

Asked by: Anonymous

Thank you for your question. sc-281692, is a Paraformaldehyde solution 4% in PBS that contains 2.5% methanol.

Answered by: Tech Support Europe

Date published: 2020-06-25

My PFA solution has been kept at 4 C for 7 months, can it still be used for fixing bacterial cells? 

Asked by: Delaney

Thank you for your question. Yes, the recommended storage temperature for this Paraformaldehyde solution 4% in PBS is 4˚C, so it should be fine.

Answered by: Tech Service

Date published: 2020-01-28

Per quanto tempo la soluzione di PFA al 4% si può tenere in frigo a +4°C se non è stata ancora aperta? quando è da considerare scaduta? invece una volta aperta come consigliate di conservarla? per quanto tempo si può tenere aperta a +4°C?

Asked by: Giorgia

Thank you for your question. We recommend solutions are used for no longer than 3 months at 4˚C from when they are made. Aliquot and freeze at -20°C for long-term storage.

Answered by: Tech Service

Date published: 2022-04-12

what is the expiration of the PFA solution if kept at 4 C.

Asked by: Uriw

Thank you for your question. We provide a warranty of one year on the viability of our products given proper storage conditions. To find out the lot-specific expiry date please contact our technical service or your local distributor for expiration information for the specific lot number of your purchased product.

Answered by: Tech Service

Date published: 2019-08-20

Is it suitable for cell fixation during intracellular staining for flow cytometry?

Asked by: Shani

Thank you for your question. We recommend using our FCM Fixation Buffer: sc-3622 for fixing an permeabilizing cells for intracellular staining.

Answered by: Technical Support

Date published: 2019-08-17

Paraformaldehyde solution 4% in PBS Questions - page 2

Rated 5 out of 5 by Samantha from PFA 4%The product appeared as was advertised, useful for tissue fixation.

Date published: 2025-09-11

Rated 5 out of 5 by Anonymous from Paraformaldehyde solution 4% 1L, great valueThe product is great, ready to use and always in stock, prompt processing and overnight shipping is awesome.

Date published: 2025-07-24

Rated 5 out of 5 by Christopher Clark from Excellent PFAThis PFA works very well for fixing our cells quickly. For both 2D and 3D cell cultures, this is the only PFA my lab uses.

Date published: 2025-06-25

Rated 5 out of 5 by xuej5 from Good productThis product, 4% PFA, is ordered routinely in our lab (once every two months). It works very well.

Date published: 2025-03-03

Rated 5 out of 5 by Mount Sinai from quick deliveryThe item is shipped very quick. Solution is good for perfusion.

Date published: 2024-10-08

Rated 5 out of 5 by Einstein from excellent productMaking paraformaldehyde solution is a time consuming and toxic thing. This product works well for our lab for years.

Date published: 2024-09-24

Rated 5 out of 5 by Steph from Well priced & ships fasrtWorks great, cheaper than Fisherbrand, and ships super fast

Date published: 2024-09-09

Rated 5 out of 5 by Cangee from Great productI have been using this product for years, and it works perfectly to fix tissue samples before paraffin or OCT embedding. It also works fine to fix cells to perform ICC.

Date published: 2023-08-21

Paraformaldehyde solution 4% in PBS Reviews - page 2

Paraformaldehyde solution 4% in PBS is rated 4.9 out of 5 by 32.

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Test	Specification	Results
Appearance	Clear, colorless, with no precipitate	Complies
pH	7.0 - 7.6 (25°C)

Paraformaldehyde solution 4% in PBS (CAS 30525-89-4)

Paraformaldehyde solution 4% in PBS (CAS 30525-89-4) References

SAMPLE Certificate of Analysis (COA)

How to request a Certificate of Analysis (COA) when it is not available online?

Paraformaldehyde solution 4% in PBS, 1 L

What is the purity of the 4% PFA in PBS?

Does the 4% solution release formaldehyde gas? or is the product off-gassed prior to shipping?

Our 4% PFA was received at an ambient temperature. How long can 4% PFA be stored at ambient room temperature?

does this product contain Methanol?

My PFA solution has been kept at 4 C for 7 months, can it still be used for fixing bacterial cells?

Per quanto tempo la soluzione di PFA al 4% si può tenere in frigo a +4°C se non è stata ancora aperta? quando è da considerare scaduta? invece una volta aperta come consigliate di conservarla? per quanto tempo si può tenere aperta a +4°C?

what is the expiration of the PFA solution if kept at 4 C.

Is it suitable for cell fixation during intracellular staining for flow cytometry?

Paraformaldehyde solution 4% in PBS (CAS 30525-89-4)

Paraformaldehyde solution 4% in PBS (CAS 30525-89-4) References

SAMPLE Certificate of Analysis (COA)

How to request a Certificate of Analysis (COA) when it is not available online?

Paraformaldehyde solution 4% in PBS, 1 L

What is the purity of the 4% PFA in PBS?

Does the 4% solution release formaldehyde gas? or is the product off-gassed prior to shipping?

Our 4% PFA was received at an ambient temperature. How long can 4% PFA be stored at ambient room temperature?

does this product contain Methanol?

My PFA solution has been kept at 4 C for 7 months, can it still be used for fixing bacterial cells?&nbsp;

Per quanto tempo la soluzione di PFA al 4% si pu&ograve; tenere in frigo a +4&deg;C se non &egrave; stata ancora aperta? quando &egrave; da considerare scaduta? invece una volta aperta come consigliate di conservarla? per quanto tempo si pu&ograve; tenere aperta a +4&deg;C?

what is the expiration of the PFA solution if kept at 4 C.

Is it suitable for cell fixation during intracellular staining for flow cytometry?

My PFA solution has been kept at 4 C for 7 months, can it still be used for fixing bacterial cells?

Per quanto tempo la soluzione di PFA al 4% si può tenere in frigo a +4°C se non è stata ancora aperta? quando è da considerare scaduta? invece una volta aperta come consigliate di conservarla? per quanto tempo si può tenere aperta a +4°C?