PAPST1 Antibody (3H5): sc-517131

PAPST1 Antibody (3H5) is a mouse monoclonal IgG2a kappa light chain antibody that detects the PAPST1 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-PAPST1 antibody (3H5) is available as a non-conjugated format. PAPST1, also known as adenosine 3′-phospho 5′-phosphosulfate transporter 1 or SLC35B2, is a crucial type III transmembrane protein composed of 432 amino acids, primarily located in the Golgi apparatus where PAPST1 facilitates the transport of 3′-phosphoadenosine 5′-phosphosulfate (PAPS) from the cytosol. This transport is vital for the sulfation of proteoglycans, glycolipids, and glycoproteins, which are essential for various biological processes, including cell signaling, structural integrity, and the regulation of cellular interactions. PAPST1 function is particularly important in the colon, where PAPST1 overexpression can enhance PAPS transport activity, thereby influencing sulfation patterns that are critical for maintaining tissue homeostasis. Additionally, in the model organism Caenorhabditis elegans, reduced PAPST1 activity has been linked to neuronal deficits, highlighting PAPST1′s role in nervous system development and the complexity of heparan sulfate patterns. Alternative splicing of the PAPST1 gene results in three distinct isoforms, each potentially contributing to diverse biological functions.

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PAPST1 Antibody (3H5) References:

1. Molecular cloning and identification of 3'-phosphoadenosine 5'-phosphosulfate transporter.  |  Kamiyama, S., et al. 2003. J Biol Chem. 278: 25958-63. PMID: 12716889
2. Inhibition of D-serine accumulation in the Xenopus oocyte by expression of the rat ortholog of human 3'-phosphoadenosine 5'-phosphosulfate transporter gene isolated from the neocortex as D-serine modulator-1.  |  Shimazu, D., et al. 2006. J Neurochem. 96: 30-42. PMID: 16277611
3. Molecular cloning and characterization of a novel 3'-phosphoadenosine 5'-phosphosulfate transporter, PAPST2.  |  Kamiyama, S., et al. 2006. J Biol Chem. 281: 10945-53. PMID: 16492677
4. Overexpression of the 3'-phosphoadenosine 5'-phosphosulfate (PAPS) transporter 1 increases sulfation of chondroitin sulfate in the apical pathway of MDCK II cells.  |  Dick, G., et al. 2008. Glycobiology. 18: 53-65. PMID: 17965432
5. Regulation of zebrafish skeletogenesis by ext2/dackel and papst1/pinscher.  |  Clément, A., et al. 2008. PLoS Genet. 4: e1000136. PMID: 18654627
6. The PAPS transporter PST-1 is required for heparan sulfation and is essential for viability and neural development in C. elegans.  |  Bhattacharya, R., et al. 2009. J Cell Sci. 122: 4492-504. PMID: 19920077
7. The 3'-phosphoadenosine 5'-phosphosulfate transporters, PAPST1 and 2, contribute to the maintenance and differentiation of mouse embryonic stem cells.  |  Sasaki, N., et al. 2009. PLoS One. 4: e8262. PMID: 20011239
8. Expression and the role of 3'-phosphoadenosine 5'-phosphosulfate transporters in human colorectal carcinoma.  |  Kamiyama, S., et al. 2011. Glycobiology. 21: 235-46. PMID: 20978009
9. MiR-22 is frequently downregulated in medulloblastomas and inhibits cell proliferation via the novel target PAPST1.  |  Xu, QF., et al. 2014. Brain Pathol. 24: 568-83. PMID: 24576181
10. PAPST1 regulates sulfation of heparan sulfate proteoglycans in epithelial MDCK II cells.  |  Dick, G., et al. 2015. Glycobiology. 25: 30-41. PMID: 25138304
11. PAPST2 Plays Critical Roles in Removing the Stress Signaling Molecule 3'-Phosphoadenosine 5'-Phosphate from the Cytosol and Its Subsequent Degradation in Plastids and Mitochondria.  |  Ashykhmina, N., et al. 2019. Plant Cell. 31: 231-249. PMID: 30464037
12. Differential genetic associations and expression of PAPST1/SLC35B2 in bipolar disorder and schizophrenia.  |  Uezato, A., et al. 2022. J Neural Transm (Vienna). 129: 913-924. PMID: 35501530