PAP-α/β/γ Antibody (D-1): sc-365607

PAP-α/β/γ Antibody (D-1) is a mouse monoclonal IgG1 antibody that detects PAP-α/β/γ of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PAP-α/β/γ (D-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PAP-α/β/γ plays a crucial role in post-transcriptional modification of eukaryotic mRNAs, specifically through poly(A) tail addition, which is essential for mRNA stability, nuclear export, and translation efficiency. This process occurs in the nucleus and involves two key steps: endoribonucleolytic cleavage of pre-mRNA at the poly(A) site, followed by poly(A) tail synthesis, catalyzed by polyadenylation polymerase (PAP). The three isoforms of PAP—PAP-α, PAP-β, and PAP-γ—exhibit distinct molecular weights and functional properties, with the amino-terminal region being critical for nonspecific polymerase activity, while both amino and carboxy termini are necessary for specific polymerase function. PAP contains a ribonucleoprotein-type RNA binding domain that facilitates primer binding, underscoring PAP′s importance in gene expression and mRNA processing regulation.

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PAP-α/β/γ Antibody (D-1) References:

1. Assignment of the human poly(A) polymerase (PAP) gene to chromosome 14q32.1-q32.2 and isolation of a polymorphic CA repeat sequence.  |  Yamauchi, T., et al. 1999. J Hum Genet. 44: 253-5. PMID: 10429366
2. A novel nuclear human poly(A) polymerase (PAP), PAP gamma.  |  Kyriakopoulou, CB., et al. 2001. J Biol Chem. 276: 33504-11. PMID: 11431479
3. Hypophosphorylation of poly(A) polymerase and increased polyadenylation activity are associated with human immunodeficiency virus type 1 Vpr expression.  |  Mouland, AJ., et al. 2002. Virology. 292: 321-30. PMID: 11878934
4. Regulation of poly(A) polymerase by 14-3-3epsilon.  |  Kim, H., et al. 2003. EMBO J. 22: 5208-19. PMID: 14517258
5. Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase.  |  Kaufmann, I., et al. 2004. EMBO J. 23: 616-26. PMID: 14749727
6. [Clinical evaluation of serum basic fetoprotein for prostatic cancer--comparative study with PAP, gamma-Sm and PSA].  |  Gotoh, A., et al. 1991. Nihon Hinyokika Gakkai Zasshi. 82: 467-72. PMID: 1712873
7. [Significance of prostatic acid phosphatase, gamma-seminoprotein and prostatic specific antigen in the urine. First report: the measurement of PAP, gamma-Sm and PA in the urine of patients with prostatic diseases].  |  Uchijima, Y., et al. 1991. Hinyokika Kiyo. 37: 1255-60. PMID: 1721770
8. Structure of yeast poly(A) polymerase in complex with a peptide from Fip1, an intrinsically disordered protein.  |  Meinke, G., et al. 2008. Biochemistry. 47: 6859-69. PMID: 18537269
9. [Prostatic acid phosphatase (PAP), gamma-seminoprotein (gamma-Sm) and prostate specific antigen (PA) in prostatic cancer].  |  Akimoto, S., et al. 1988. Hinyokika Kiyo. 34: 1389-96. PMID: 2461644
10. Multiple forms of poly(A) polymerases in human cells.  |  Thuresson, AC., et al. 1994. Proc Natl Acad Sci U S A. 91: 979-83. PMID: 8302877
11. Tat-dependent occlusion of the HIV poly(A) site.  |  Weichs an der Glon, C., et al. 1993. EMBO J. 12: 2119-28. PMID: 8491200
12. Binding of factor VIIa to tissue factor induces alterations in gene expression in human fibroblast cells: up-regulation of poly(A) polymerase.  |  Pendurthi, UR., et al. 1997. Proc Natl Acad Sci U S A. 94: 12598-603. PMID: 9356495

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PAP-α/β/γ Antibody (D-1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PAP-α/β/γ Antibody (D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PAP-α/β/γ Antibody (D-1) Specifications

Alternate Product Name: PAP-α/β/γ

Product Clone Name: D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ lambda light chain

Amino Acids: 85-117

Epitope Target: PAP-α

Epitope Species: human

Product Reactivity/Detection: PAP-α, PAP-β and PAP-γ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PAP-α/β/γ Antibody (D-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin