PAO Antibody (C-3): sc-166185

PAO Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PAO protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PAO (C-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PAO protein plays a crucial role in regulating polyamine metabolism, specifically in the back-conversion pathway where PAO catalyzes the oxidation of acetylated spermine and spermidine, leading to the production of spermidine and putrescine. This regulation is vital as polyamines are essential for cellular growth and function, and their dysregulation can contribute to tumorigenesis. Furthermore, PAO gene encodes several transcript variants that produce four active isoenzymes, with the longest isoenzyme, PAOh1, representing a novel addition to the polyamine metabolic pathway and potentially serving as a target for antineoplastic drug development. By modulating intracellular polyamine concentrations, PAO may influence cellular sensitivity to antitumor polyamine analogs, making PAO protein a significant target in cancer research and therapeutic strategies.

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PAO Antibody (C-3) References:

1. Identification and characterization of a novel flavin-containing spermine oxidase of mammalian cell origin.  |  Vujcic, S., et al. 2002. Biochem J. 367: 665-75. PMID: 12141946
2. Properties of purified recombinant human polyamine oxidase, PAOh1/SMO.  |  Wang, Y., et al. 2003. Biochem Biophys Res Commun. 304: 605-11. PMID: 12727196
3. Genomic identification and biochemical characterization of a second spermidine/spermine N1-acetyltransferase.  |  Chen, Y., et al. 2003. Biochem J. 373: 661-7. PMID: 12803540
4. Spermine oxidase SMO(PAOh1), Not N1-acetylpolyamine oxidase PAO, is the primary source of cytotoxic H2O2 in polyamine analogue-treated human breast cancer cell lines.  |  Pledgie, A., et al. 2005. J Biol Chem. 280: 39843-51. PMID: 16207710
5. Regulation of c-fos and c-jun protooncogene expression by the Ca(2+)-ATPase inhibitor thapsigargin.  |  Schönthal, A., et al. 1991. Proc Natl Acad Sci U S A. 88: 7096-100. PMID: 1714585
6. Chronic sub-lethal oxidative stress by spermine oxidase overactivity induces continuous DNA repair and hypersensitivity to radiation exposure.  |  Bianchi, M., et al. 2007. Biochim Biophys Acta. 1773: 774-83. PMID: 17363080
7. Increased spermine oxidase expression in human prostate cancer and prostatic intraepithelial neoplasia tissues.  |  Goodwin, AC., et al. 2008. Prostate. 68: 766-72. PMID: 18302221
8. Nuclear localization of human spermine oxidase isoforms - possible implications in drug response and disease etiology.  |  Murray-Stewart, T., et al. 2008. FEBS J. 275: 2795-806. PMID: 18422650
9. Effect of expression of human spermidine/spermine N1-acetyltransferase in Escherichia coli.  |  Parry, L., et al. 1995. Biochemistry. 34: 2701-9. PMID: 7873553

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PAO Antibody (C-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-PAO Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PAO Antibody (C-3) Specifications

Alternate Product Name: PAO

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 197-281

Epitope Target: PAO1

Epitope Species: human

Product Reactivity/Detection: PAO1, PAO2, PAO4 and PAO5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for PAO Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin