pADPr Antibody (10H): sc-56198

pADPr Antibody (10H) is a mouse monoclonal IgG3 antibody that detects pADPr in non-species reactive applications such as western blotting (WB), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-pADPr antibody (10H) is available as a non-conjugated monoclonal isotype antibody. Poly(ADP-ribosylation) is a crucial post-translational modification that plays a significant role in cellular responses to DNA damage, helping to protect proliferating cells from apoptosis. The process is primarily mediated by the poly(ADP-ribose) polymerase (PARP) superfamily of enzymes, which utilize NAD+ as a substrate to transfer ADP-ribose units onto target proteins, resulting in the formation of poly(ADP-ribose) chains. These chains can either exist independently or be covalently attached to proteins, thereby modulating their function and promoting cell survival. The dynamic nature of pADPr chains is essential for various cellular processes, including cell-cycle regulation and the maintenance of genomic integrity, making the study of this modification vital for understanding cellular responses to stress and the mechanisms underlying various diseases.

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pADPr Antibody (10H) References:

1. Overexpression of dominant negative PARP interferes with tumor formation of HeLa cells in nude mice: evidence for increased tumor cell apoptosis in vivo.  |  Hans, MA., et al. 1999. Oncogene. 18: 7010-5. PMID: 10597301
2. Analysis of ADP-ribose polymer sizes in intact cells.  |  Gagné, JP., et al. 2001. Mol Cell Biochem. 224: 183-5. PMID: 11693195
3. B-aggressive lymphoma family proteins have unique domains that modulate transcription and exhibit poly(ADP-ribose) polymerase activity.  |  Aguiar, RC., et al. 2005. J Biol Chem. 280: 33756-65. PMID: 16061477
4. CDK-dependent activation of poly(ADP-ribose) polymerase member 10 (PARP10).  |  Chou, HY., et al. 2006. J Biol Chem. 281: 15201-7. PMID: 16455663
5. The expanding role of poly(ADP-ribose) metabolism: current challenges and new perspectives.  |  Gagné, JP., et al. 2006. Curr Opin Cell Biol. 18: 145-51. PMID: 16516457
6. Collaborator of Stat6 (CoaSt6)-associated poly(ADP-ribose) polymerase activity modulates Stat6-dependent gene transcription.  |  Goenka, S., et al. 2007. J Biol Chem. 282: 18732-9. PMID: 17478423
7. The diverse biological roles of mammalian PARPS, a small but powerful family of poly-ADP-ribose polymerases.  |  Hassa, PO. and Hottiger, MO. 2008. Front Biosci. 13: 3046-82. PMID: 17981777
8. Poly(ADP-ribose) polymerase 1 promotes tumor cell survival by coactivating hypoxia-inducible factor-1-dependent gene expression.  |  Elser, M., et al. 2008. Mol Cancer Res. 6: 282-90. PMID: 18314489
9. Poly (ADP-ribose) polymerase activity regulates apoptosis in HeLa cells after alkylating DNA damage.  |  Liu, X., et al. 2008. Cancer Biol Ther. 7: 934-41. PMID: 18376143
10. Noncovalent pADPr interaction with proteins and competition with RNA for binding to proteins.  |  Ji, Y. 2011. Methods Mol Biol. 780: 83-91. PMID: 21870256
11. The immunodetection of pADPr in Drosophila tissues: immunostaining of thick sections; immuno-EM.  |  Jarnik, M. 2011. Methods Mol Biol. 780: 401-11. PMID: 21870274
12. Detecting and Quantifying pADPr In Vivo.  |  Lamade, AM., et al. 2023. Methods Mol Biol. 2609: 23-42. PMID: 36515827

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-pADPr Antibody (10H) has 68 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

pADPr Antibody (10H) Specifications

Alternate Product Name: pADPr

Product Clone Name: 10H

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃

Amino Acids:

Epitope Target: pADPr

Epitope Species: NSR

Product Reactivity/Detection: poly(ADP-ribose) polymer (pADPr) synthesized by a variety of pADPr polymerase (PARP)-related enzymes including PARP-1, -2, -3, tankyrase, vPARP, sPARP and others.

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG₃ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 68 citations in various scientific publications

Available Conjugates/Forms for pADPr Antibody (10H): N/A