PAcP Double Nickase Plasmid (h): sc-405118-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PAcP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PAcP Double Nickase Plasmid (h) and PAcP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ACPP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PAcP Antibody (PAP-1): sc-69858
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAcP Double Nickase Plasmid (h) | sc-405118-NIC | 20 µg | $410.00 | |||
PAcP Double Nickase Plasmid (h2) | sc-405118-NIC-2 | 20 µg | $410.00 |
ACPP encodes prostatic acid phosphatase (PAcP), a secreted and intracellular acid phosphatase that hydrolyzes phosphate monoesters and can function as a protein tyrosine phosphatase in prostate epithelial cells. PAcP activity influences phosphorylation-dependent signaling and cellular differentiation programs, with reported connections to growth factor receptor and androgen-responsive pathways that shape prostate cell homeostasis. Altered ACPP expression and PAcP enzymatic function have been studied in the context of prostate cancer biology, including changes in signaling output and tumor cell phenotype. As a prostate-enriched marker with mechanistic links to phosphoregulation, ACPP is frequently used to interrogate lineage identity, signaling control, and cancer-associated pathway remodeling.
PAcP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ACPP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ACPP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ACPP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ACPP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.