Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
p68 RNA Helicase Double Nickase Plasmid (h): sc-402401-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p68 RNA Helicase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- p68 RNA Helicase Double Nickase Plasmid (h) and p68 RNA Helicase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DDX5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p68 RNA Helicase Antibody (D-7): sc-365164
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p68 RNA Helicase Double Nickase Plasmid (h) | sc-402401-NIC | 20 µg | $410.00 | |||
p68 RNA Helicase Double Nickase Plasmid (h2) | sc-402401-NIC-2 | 20 µg | $410.00 |
DDX5 encodes p68 RNA helicase, a DEAD-box ATP-dependent RNA helicase that remodels RNA and ribonucleoprotein complexes to regulate pre-mRNA splicing, rRNA processing, transcription, and microRNA biogenesis. p68 functions as a multifunctional co-regulator of transcription factors and chromatin-associated complexes, linking RNA metabolism to cell-cycle progression, DNA damage responses, and stress signaling. It participates in pathways controlling RNA polymerase II transcriptional programs and RNA quality control, influencing cellular differentiation and proliferation. Dysregulated DDX5 activity and expression have been associated with altered oncogenic signaling and transcriptome instability, making it a common target in mechanistic studies of tumor biology and RNA processing defects.
p68 RNA Helicase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DDX5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DDX5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DDX5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DDX5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.