P5CRL Antibody (E-63): sc-100487

P5CRL Antibody (E-63) is a mouse monoclonal IgG2a kappa light chain antibody that detects P5CRL protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-P5CRL antibody (E-63) is available as a non-conjugated format. P5CRL protein, also known as pyrroline-5-carboxylate reductase-like, is crucial in proline biosynthesis, a process vital for maintaining cellular homeostasis and responding to stress. Proline plays a significant role in protein folding, stability, and structure, and its presence can act as a sequence-recognition motif in various proteins. Additionally, proline functions as a non-enzymatic antioxidant, helping to mitigate damage from reactive oxygen species (ROS) across different organisms, including microorganisms, animals, and plants. P5CRL protein consists of 274 amino acids and belongs to the pyrroline-5-carboxylate reductase family, functioning as a homodecamer formed by five homodimers. This structural arrangement is essential for enzymatic activity, as P5CRL allows for efficient catalysis in the final step of proline biosynthesis, where pyrroline-5-carboxylate reductase (P5CR) reduces aldehyde dehydrogenase 4A1 (ALDH4A1) to proline using NAD(P)H as a cofactor. P5CRL′s participation in these critical biochemical pathways underscores its importance in cellular function and stress response, making P5CRL monoclonal antibody (E-63) a valuable tool for researchers studying proline metabolism and its implications in health and disease.

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P5CRL Antibody (E-63) References:

1. Molecular cloning and evidence for osmoregulation of the delta 1-pyrroline-5-carboxylate reductase (proC) gene in pea (Pisum sativum L.).  |  Williamson, CL. and Slocum, RD. 1992. Plant Physiol. 100: 1464-70. PMID: 11537868
2. Purification and characterization of a functionally active Mycobacterium tuberculosis pyrroline-5-carboxylate reductase.  |  Yang, Y., et al. 2006. Protein Expr Purif. 45: 241-8. PMID: 16199181
3. Crystal structures of delta1-pyrroline-5-carboxylate reductase from human pathogens Neisseria meningitides and Streptococcus pyogenes.  |  Nocek, B., et al. 2005. J Mol Biol. 354: 91-106. PMID: 16233902
4. Purification, characterization, and crystallization of human pyrroline-5-carboxylate reductase.  |  Meng, Z., et al. 2006. Protein Expr Purif. 49: 83-7. PMID: 16600630
5. Crystal structure of human pyrroline-5-carboxylate reductase.  |  Meng, Z., et al. 2006. J Mol Biol. 359: 1364-77. PMID: 16730026
6. Proline modulates the intracellular redox environment and protects mammalian cells against oxidative stress.  |  Krishnan, N., et al. 2008. Free Radic Biol Med. 44: 671-81. PMID: 18036351
7. Tailoring the structure of aminobisphosphonates to target plant P5C reductase.  |  Forlani, G., et al. 2008. J Agric Food Chem. 56: 3193-9. PMID: 18399639

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-P5CRL Antibody (E-63) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-P5CRL Antibody (E-63) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

P5CRL Antibody (E-63) Specifications

Alternate Product Name: P5CRL

Product Clone Name: E-63

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 32-132

Epitope Target: P5CRL

Epitope Species: human

Product Reactivity/Detection: P5CRL

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for P5CRL Antibody (E-63): N/A