p53 Antibody (F-8): sc-374087

p53 Antibody (F-8) mouse monoclonal IgG1 kappa light chain antibody specific for an epitope mapping between amino acids 367-390 at the C-terminus of p53 of mouse origin. p53 monoclonal antibody (F-8) is reactive with mouse, rat, and human species and is recommended for various applications such as western blotting, immunoprecipitation, immunofluorescence, and enzyme-linked immunosorbent assay. The p53 protein, encoded by the TP53 gene, serves as a critical tumor suppressor that functions primarily as a transcription factor that regulates key processes, including DNA repair, apoptosis, cell cycle arrest, and senescence. Upon DNA damage, p53 can halt the cell cycle at the G1/S checkpoint, allowing time for repair mechanisms to correct the damage. If the damage is irreparable, p53 can trigger apoptosis, preventing the propagation of mutated cells. Research with p53 antibodies, particularly the p53 Antibody (F-8) monoclonal antibody, has proven invaluable in studying the localization and expression of p53 in cells. p53 mouse antibody (F-8) specifically binds to p53, allowing researchers to investigate p53′s involvement in cancer development and progression. Given that mutations in the TP53 gene are present in nearly half of all human cancers, restoring or modulating p53 function remains a key focus of cancer therapy research. Mutations in p53, especially within its DNA-binding domain, often lead to a loss of tumor suppression and can confer oncogenic properties that promote tumor growth. Anti-p53 Antibody (F-8) plays a pivotal role in cancer research, facilitating the identification and study of mutant p53 in various cancer types. By understanding how different mutations impact p53′s structure and function, scientists are better equipped to develop targeted therapies aimed at reactivating p53 or inhibiting its negative regulators like MDM2.

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p53 Antibody (F-8) References:

1. 9-Hydroxyellipticine alters the conformation and DNA binding characteristics of mutated p53 protein.  |  Sugikawa, E., et al. 2001. Anticancer Res. 21: 2671-5. PMID: 11724337
2. The tumor suppressor gene TP53: implications for cancer management and therapy.  |  Seemann, S., et al. 2004. Crit Rev Clin Lab Sci. 41: 551-83. PMID: 15603511
3. Single chain antibody against the common epitope of mutant p53 restores wild-type activity to mutant p53 protein.  |  Orgad, S., et al. 2005. FEBS Lett. 579: 5609-15. PMID: 16213502
4. Toward Understanding the Molecular Recognition of Albumin by p53-Activating Stapled Peptide ATSP-7041.  |  Tiwari, G. and Verma, CS. 2017. J Phys Chem B. 121: 657-670. PMID: 28048940
5. Forms and functions of p53.  |  Milner, J. 1994. Semin Cancer Biol. 5: 211-9. PMID: 7948949
6. Tumour suppressor genes and molecular chaperones.  |  Lane, DP., et al. 1993. Philos Trans R Soc Lond B Biol Sci. 339: 369-72; discussion 372-3. PMID: 8493291
7. Antisense p53 provokes changes in HeLa cell growth and morphology.  |  Iotsova, V. and Stehelin, D. 1995. Eur J Cell Biol. 68: 122-32. PMID: 8575459
8. Serological and immunohistochemical determinations of p53 protein in diagnosis of endometrial cancer: a comparative study.  |  Ben-Hur, H., et al. 1997. Eur J Gynaecol Oncol. 18: 400-6. PMID: 9378162
9. Function and conformation of wild-type p53 protein are influenced by mutations in bovine leukemia virus-induced B-cell lymphosarcoma.  |  Tajima, S., et al. 1998. Virology. 243: 735-46. PMID: 9540787
10. Curcumin induces a p53-dependent apoptosis in human basal cell carcinoma cells.  |  Jee, SH., et al. 1998. J Invest Dermatol. 111: 656-61. PMID: 9764849

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.