p53 Antibody (A-1): sc-393031

p53 (A-1) Antibody is an IgG1 kappa light chain mouse monoclonal antibody, also designated TP53, that detects the p53 protein of mouse, rat, and human origin by western blotting, immunoprecipitation, immunofluorescence, and enzyme-linked immunosorbent assay. p53 monoclonal antibody (A-1) is available as the non-conjugated anti-p53 antibody form, as well as multiple conjugated forms of anti-p53 antibody (A-1), including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. The p53 protein, encoded by the TP53 gene in humans, is a pivotal tumor suppressor and has an important role in maintaining genomic stability. As a transcription factor, p53 regulates the expression of genes involved in essential cellular processes such as cell cycle arrest, DNA repair, apoptosis, and senescence. Upon detecting DNA damage, p53 can induce cell cycle arrest at the G1/S checkpoint, allowing time for repair before replication proceeds. If the damage is irreparable, p53 can initiate apoptosis to eliminate the potentially harmful cell, preventing the propagation of mutations. The activity of p53 is tightly regulated through post-translational modifications like phosphorylation, acetylation, and ubiquitination, which affect its stability and function. MDM2 is a key negative regulator that promotes p53 degradation via the ubiquitin-proteasome pathway. Mutations in the TP53 gene are found in approximately 50% of human cancers, making it one of the most frequently mutated genes associated with cancer. These mutations often occur in the DNA-binding domain of p53, leading to a loss of its tumor-suppressing capabilities. Some mutant forms not only lose their protective functions but also gain oncogenic properties that promote tumor development. Understanding the structural domains of p53, the transactivation domain, DNA-binding domain, and oligomerization domain, is essential as each is critical for its function. Research on p53 has significant implications for cancer therapy, with strategies aiming to restore its normal function in tumors by developing small molecules to reactivate mutant p53 or inhibit its negative regulators like MDM2. Animal models, such as p53 knockout mice, along with p53 antibodies like the p53 mouse monoclonal antibody (A-1), have been instrumental in advancing our overall understanding of the protein′s functions and importance by offering valuable insights into cellular stress responses and tumor suppression mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p53 Antibody (A-1) References:

1. p53 and its isoforms in cancer.  |  Bourdon, JC. 2007. Br J Cancer. 97: 277-82. PMID: 17637683
2. Versatile functions of p53 protein in multicellular organisms.  |  Chumakov, PM. 2007. Biochemistry (Mosc). 72: 1399-421. PMID: 18282133
3. Detection of p53 protein aggregation in cancer cell lines and tumor samples.  |  Yang-Hartwich, Y., et al. 2015. Methods Mol Biol. 1219: 75-86. PMID: 25308263
4. Small-molecule stabilization of the p53 - 14-3-3 protein-protein interaction.  |  Doveston, RG., et al. 2017. FEBS Lett. 591: 2449-2457. PMID: 28640363
5. Loss of p53 protein strikes a nerve to aid tumour growth.  |  Napoli, M. and Flores, ER. 2020. Nature. 578: 367-369. PMID: 32066916
6. Promising New Tools for Targeting p53 Mutant Cancers: Humoral and Cell-Based Immunotherapies.  |  Chasov, V., et al. 2021. Front Immunol. 12: 707734. PMID: 34484205
7. [P53 and cancers].  |  May, P. and May, E. 1995. Pathol Biol (Paris). 43: 165-73. PMID: 7675543
8. Increased p53 protein associated with aging in human diploid fibroblasts.  |  Kulju, KS. and Lehman, JM. 1995. Exp Cell Res. 217: 336-45. PMID: 7698234
9. p53 and human cancers.  |  Lane, DP. 1994. Br Med Bull. 50: 582-99. PMID: 7987642
10. On the expression of the p53 protein in human cancer.  |  Lane, DP. 1994. Mol Biol Rep. 19: 23-9. PMID: 8170465
11. The p53 protein is an unusually shaped tetramer that binds directly to DNA.  |  Friedman, PN., et al. 1993. Proc Natl Acad Sci U S A. 90: 3319-23. PMID: 8475074
12. Non-p53 p53RE binding protein, a human transcription factor functionally analogous to P53.  |  Zeng, X., et al. 1998. Proc Natl Acad Sci U S A. 95: 6681-6. PMID: 9618472

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p53 Antibody (A-1) has 66 citations in a variety of scientific publications.

Primary antibodies like Anti-p53 Antibody (A-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p53 Antibody (A-1) Specifications

Alternate Product Name: TP53

Product Clone Name: A-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 353-391

Epitope Target: p53

Epitope Species: rat

Product Reactivity/Detection: p53

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 66 citations in various scientific publications

Available Conjugates/Forms for p53 Antibody (A-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.