p53 Antibody (3H2821): sc-56179

p53 Antibody (3H2821) is a mouse monoclonal antibody IgG1 kappa light chain raised against SVA31E7 mouse SV40-transformed cell line and is specifically designed to detect the p53 protein in mouse and rat species. The p53 protein plays a pivotal role in cellular stress response by upregulating genes involved in growth arrest, apoptosis, and other cell cycle control mechanisms, ultimately influencing processes such as programmed cell death and cell differentiation. Normally localized to the nucleus, p53 can be chaperoned to the cytoplasm by MDM2, an E3 ubiquitin ligase that targets p53 for degradation. MDM2 is itself upregulated by active p53, creating a regulatory feedback loop. The activity of p53 is modulated by various post-translational modifications, such as phosphorylation and acetylation, which can alter its DNA-binding conformation. Mutations in the DNA-binding domain, particularly between amino acids 110-286, are known to compromise its interaction with DNA and are linked to numerous cancers. Anti-p53 monoclonal antibody (3H2821) recognizes a specific epitope of p53 that is unstable in the absence of SV40 large T antigen. In SV40 transformed cells, p53 becomes tightly complexed with the large T antigen, leading to significantly increased stability. In transformed murine 3T3 cells, p53 levels can be up to 1,000-fold higher than in the parental, untransformed cells. p53 mouse monoclonal antibody (3H2821) is particularly useful for studying the functional dynamics of p53 in the context of viral transformation and tumor biology.

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p53 Antibody (3H2821) References:

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2. Generation of p53 suppressor peptide from the fragment of p53 protein.  |  Mittelman, JM. and Gudkov, AV. 1999. Somat Cell Mol Genet. 25: 115-28. PMID: 11441532
3. Expression of p53 protein in colorectal cancer and its relationship to short-term prognosis.  |  Yamaguchi, A., et al. 1992. Cancer. 70: 2778-84. PMID: 1451055
4. Human tumor suppressor p53 and DNA viruses.  |  Collot-Teixeira, S., et al. 2004. Rev Med Virol. 14: 301-19. PMID: 15334538
5. Advantage of a baculovirus expression system for protein-protein interaction studies. Involvement of posttranslational phosphorylation in the interaction between wt p53 protein and poly(ADP-ribose) polymerase-1.  |  Schmid, G., et al. 2005. Acta Biochim Pol. 52: 713-9. PMID: 16082409
6. Posttranslational modification of p53: cooperative integrators of function.  |  Meek, DW. and Anderson, CW. 2009. Cold Spring Harb Perspect Biol. 1: a000950. PMID: 20457558
7. Characterization of p53 Family Homologs in Evolutionary Remote Branches of Holozoa.  |  Bartas, M., et al. 2019. Int J Mol Sci. 21: PMID: 31861340
8. A proteolytic fragment from the central region of p53 has marked sequence-specific DNA-binding activity when generated from wild-type but not from oncogenic mutant p53 protein.  |  Bargonetti, J., et al. 1993. Genes Dev. 7: 2565-74. PMID: 8276239
9. A study on p53 protein and anti-p53 antibodies in the sera of patients with oesophageal cancer.  |  Sobti, RC. and Parashar, K. 1998. Mutat Res. 422: 271-7. PMID: 9838161
10. Clinical applications of detecting dysfunctional p53 tumor suppressor protein.  |  Baas, IO., et al. 1999. Histol Histopathol. 14: 279-84. PMID: 9987672

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.