p38 alpha Antibody (27) recommended for detection of p38α of mouse, rat, human and canine origin by WB and IF

p38α Antibody (27): sc-136210

p38α Antibody (27) is rated 5.0 out of 5 by 5.
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Datasheets

    • p38α Antibody (27) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against amino acids 243-355 of p38α of human origin
    • recommended for detection of p38α of mouse, rat, human and canine origin by WB and IF
    • See p38α/β (A-12): sc-7972 for p38α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended fixation method for immunofluorescence staining with p38α (27): sc-136210 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Very strong signal Antibody works really well. 20 µg of protein loaded and a very strong signal with a 1:1000 dilution of the antibody.
Date published: 2018-12-21
Rated 5 out of 5 by from specific signal Signal not as strong as against human but still very good, non-specific bands are light and do not interfere with specific band
Date published: 2017-02-12
Rated 5 out of 5 by from GOOD antibody I recommend this antibody!! The antibody was able to detect endogenous protein with WB in 293T, HELA whole cell lysates.I used it at a dilution of 1:500 for WB, and the results were great!
Date published: 2016-12-07
Rated 5 out of 5 by from Great WB data obtained using Jurkat whole Great WB data obtained using Jurkat whole cell lysate. -SCBT QC
Date published: 2015-03-16
Rated 5 out of 5 by from Good WB publishable data gathered with mouse Good WB publishable data gathered with mouse leukemic monocytic macrophage cell line. -SCBT Publication Review
Date published: 2014-12-08
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