Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
p38 alpha MAPK14 Double Nickase Plasmid (m): sc-424051-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p38 alpha MAPK14 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- p38 alpha MAPK14 Double Nickase Plasmid (m) and p38 alpha MAPK14 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Mapk14. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p38 alpha MAPK14 Antibody (F-9): sc-271120
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p38 alpha MAPK14 Double Nickase Plasmid (m) | sc-424051-NIC | 20 µg | $410.00 | |||
p38 alpha MAPK14 Double Nickase Plasmid (m2) | sc-424051-NIC-2 | 20 µg | $410.00 |
Mouse Mapk14 encodes p38α (MAPK14), a stress-activated serine/threonine kinase that integrates inflammatory cytokines, oxidative stress, and environmental stimuli to shape cellular responses. p38α functions in the MAPK signaling cascade, regulating phosphorylation programs that control transcription, mRNA stability, apoptosis, differentiation, and cell-cycle checkpoints through downstream targets such as MAPKAPK2/3 and transcription factors including ATF2 and CREB. In immune and stromal compartments, MAPK14 contributes to cytokine production and innate immune signaling, linking cellular stress responses to tissue homeostasis. Dysregulated p38α activity has been implicated in inflammation-associated pathophysiology, neuroinflammatory processes, and tumor microenvironment biology, supporting its use in mechanistic studies across diverse mouse disease models.
p38 alpha MAPK14 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Mapk14 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Mapk14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Mapk14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Mapk14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.