Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
P2X7 Double Nickase Plasmid (h): sc-400780-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- P2X7 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- P2X7 Double Nickase Plasmid (h) and P2X7 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting P2RX7. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: P2X7 Antibody (D-1): sc-514962
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P2X7 Double Nickase Plasmid (h) | sc-400780-NIC | 20 µg | $410.00 | |||
P2X7 Double Nickase Plasmid (h2) | sc-400780-NIC-2 | 20 µg | $410.00 |
Human P2RX7 encodes the P2X7 purinergic receptor, an ATP-gated cation channel that couples extracellular danger signals to ion flux, membrane permeabilization, and downstream inflammatory responses. P2X7 activation promotes Ca²⁺/Na⁺ influx and K⁺ efflux, supporting NLRP3 inflammasome activation, IL-1β maturation, and pyroptosis, while also influencing ROS generation, autophagy, and metabolic adaptation. Through these processes, P2X7 links purinergic signaling to immune cell activation, cytokine release, and cell death pathways in myeloid and lymphoid compartments. Dysregulated P2RX7 activity and signaling has been associated with chronic inflammation, neuroinflammatory processes, and tumor–immune microenvironment modulation, making it a useful target for mechanistic studies of innate immunity and stress signaling.
P2X7 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the P2RX7 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within P2RX7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt P2RX7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of P2RX7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.