p22-phox Antibody (CS9) recommended for detection of p22-phox of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

p22-phox Antibody (CS9): sc-130551

p22-phox Antibody (CS9) is rated 4.5 out of 5 by 4.
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Datasheets

    • p22-phox Antibody (CS9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 165-169 near the C-terminus of p22-phox of human origin
    • Anti-p22-phox Antibody (CS9) is recommended for detection of p22-phox of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-p22-phox Antibody (CS9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p22-phox (CS9): sc-130551.

I am using p22-phox (CS9): sc-130551 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 5
Date published: 2017-02-09
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Rated 5 out of 5 by from Preferred choice for IHC(P) p22-phox Antibody (CS9) is the preferred choice for IHC(P). Alternate clones available are preferred for Western blot, but the CS9 clone gives excellent results in FFPE.
Date published: 2017-01-21
Rated 5 out of 5 by from Strong band in western blot analysis of p22 Strong band in western blot analysis of p22-phox expression in THP-1 whole cell lysate. -SCBT QC
Date published: 2015-04-16
Rated 4 out of 5 by from Published WB data with human and hamster H661 Published WB data with human and hamster H661, CHO-91-47-67, CHO-K1 - SCBT Publication Review
Date published: 2015-04-03
Rated 4 out of 5 by from Produced good immunoperoxidase cytoplasmic Produced good immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2014-01-19
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