p150 Antibody (F-7): sc-518227
- p150 Antibody (F-7) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 840-862 of p150 of human origin
- recommended for detection of p150 of human origin by WB, IP, IF and ELISA
- Anti-p150 Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for p150 Antibody (F-7) for WB applications. This reagent is now offered in a bundle with p150 Antibody (F-7) (see ordering information below).
QUICK LINKS
SEE ALSO...
p150 Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects p150 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-p150 antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p150 plays a crucial role in cellular sorting and delivery of proteins to appropriate organelles, which is vital for maintaining normal cellular function. Specifically, p150 is the human homolog of yeast Vps15p protein, which is integral to the protein trafficking system that transports vacuolar proteins from the Golgi network to the vacuole, where proteins undergo essential post-translational modifications. The interaction between p150 and Vps34p, a key component of this system, is particularly important as Vps15p enhances Vps34p′s kinase activity and recruits Vps34p to the Golgi membrane. Additionally, p150 undergoes post-translational modifications, including myristoylation, which can influence localization and function within the cell, making p150 a critical player in protein sorting and a significant target for research into cellular processes and potential therapeutic interventions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p150 Antibody (F-7) References:
- Protein targeting to the yeast vacuole. | Rothman, JH., et al. 1989. Trends Biochem Sci. 14: 347-50. PMID: 2529676
- A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system. | Volinia, S., et al. 1995. EMBO J. 14: 3339-48. PMID: 7628435
- Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast. | Stack, JH., et al. 1995. J Cell Biol. 129: 321-34. PMID: 7721937
- Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities. | Stack, JH. and Emr, SD. 1994. J Biol Chem. 269: 31552-62. PMID: 7989323
- Receptor-mediated protein sorting to the vacuole in yeast: roles for a protein kinase, a lipid kinase and GTP-binding proteins. | Stack, JH., et al. 1995. Annu Rev Cell Dev Biol. 11: 1-33. PMID: 8689553
- Characterization of p150, an adaptor protein for the human phosphatidylinositol (PtdIns) 3-kinase. Substrate presentation by phosphatidylinositol transfer protein to the p150.Ptdins 3-kinase complex. | Panaretou, C., et al. 1997. J Biol Chem. 272: 2477-85. PMID: 8999962
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p150 Antibody (F-7) | sc-518227 | 200 µg/ml | $322.00 | |||
p150 Antibody (F-7): m-IgG1 BP-HRP Bundle | sc-551461 | 200 µg Ab; 20 µg BP | $361.00 | |||
p150 Antibody (F-7) AC | sc-518227 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p150 Antibody (F-7) HRP | sc-518227 HRP | 200 µg/ml | $322.00 | |||
p150 Antibody (F-7) FITC | sc-518227 FITC | 200 µg/ml | $336.00 | |||
p150 Antibody (F-7) PE | sc-518227 PE | 200 µg/ml | $349.00 | |||
p150 Antibody (F-7) Alexa Fluor® 488 | sc-518227 AF488 | 200 µg/ml | $364.00 | |||
p150 Antibody (F-7) Alexa Fluor® 546 | sc-518227 AF546 | 200 µg/ml | $364.00 | |||
p150 Antibody (F-7) Alexa Fluor® 594 | sc-518227 AF594 | 200 µg/ml | $364.00 | |||
p150 Antibody (F-7) Alexa Fluor® 647 | sc-518227 AF647 | 200 µg/ml | $364.00 | |||
p150 Antibody (F-7) Alexa Fluor® 680 | sc-518227 AF680 | 200 µg/ml | $364.00 | |||
p150 Antibody (F-7) Alexa Fluor® 790 | sc-518227 AF790 | 200 µg/ml | $364.00 |