Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
p120 Double Nickase Plasmid (h): sc-400943-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p120 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- p120 Double Nickase Plasmid (h) and p120 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CTNND1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p120 Antibody (6H11): sc-23873
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p120 Double Nickase Plasmid (h) | sc-400943-NIC | 20 µg | $410.00 | |||
p120 Double Nickase Plasmid (h2) | sc-400943-NIC-2 | 20 µg | $410.00 |
CTNND1 encodes p120-catenin (p120), an armadillo repeat protein that binds and stabilizes classical cadherins at adherens junctions to regulate cell–cell adhesion and epithelial barrier integrity. By modulating cadherin turnover, p120 influences junctional remodeling, cytoskeletal dynamics, and Rho family GTPase signaling that coordinates cell polarity and motility. CTNND1 also interfaces with Wnt/β-catenin and growth factor–responsive pathways through its roles in adherens junction homeostasis and transcriptional regulation via Kaiso. Dysregulated CTNND1/p120 function and altered junctional signaling are associated with abnormal epithelial organization and invasive phenotypes studied across cancer biology and developmental disorders.
p120 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CTNND1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CTNND1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CTNND1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CTNND1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.