Phosphotyrosine Antibody (PY20): sc-508

p-Tyr Antibody (PY20) is a mouse monoclonal IgG2b kappa light chain antibody that detects phosphotyrosine across multiple species through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-phosphotyrosine antibody (PY20) comes in non-conjugated form and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Phosphotyrosine plays a crucial role in cellular signaling, particularly in regulating protein tyrosine kinases, which mediate signal transduction pathways. These kinases fall into two main groups: receptor tyrosine kinases and non-receptor tyrosine kinases, with Src kinases being a key example of the latter. Src kinases typically localize to the inner plasma membrane, acting as signal transducers alongside surface receptors lacking intracellular catalytic domains. Receptor tyrosine kinases include over fifty members across fourteen families, and ligand-induced tyrosine phosphorylation triggers receptor dimerization and autophosphorylation of specific phosphotyrosine residues within cytoplasmic domains. These phosphorylated residues serve as binding sites for cytoplasmic signaling molecules, influencing various cellular processes. Phosphotyrosine monoclonal antibody (PY20) serves as a valuable tool for characterizing and purifying phosphotyrosine-containing proteins, advancing our understanding of cellular signaling and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Phosphotyrosine Antibody (PY20) has 1,325 citations in a variety of scientific publications.

Primary antibodies like Anti-Phosphotyrosine Antibody (PY20) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Phosphotyrosine Antibody (PY20) Specifications

Alternate Product Name: phosphotyrosine

Product Clone Name: PY20

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: N/A

Epitope Target: p-Tyr

Epitope Species:

Product Reactivity/Detection: phosphotyrosine-containing proteins

Species Reactivity/Detection: broad species

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1,325 citations in various scientific publications

Available Conjugates/Forms for Phosphotyrosine Antibody (PY20): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Phosphotyrosine antibody is Phosphotyrosine Antibody (PY99): sc-7020.