Phosphotyrosine Antibody (2C8): sc-81529

p-Tyr Antibody (2C8) is a mouse monoclonal IgG1 antibody that detects phosphotyrosine in various species by western blotting (WB) and immunoprecipitation (IP). p-Tyr (2C8) antibody is available in a non-conjugated form, specifically designed for the detection of phosphotyrosine-containing proteins, which play a crucial role in cellular signaling pathways. Phosphotyrosine serves as a key regulatory mechanism in signal transduction, influencing processes such as cell growth, differentiation, and metabolism. Phosphotyrosine is generated through the action of protein tyrosine kinases, which can be categorized into receptor tyrosine kinases and non-receptor tyrosine kinases, with Src kinases being a prominent example of the latter. These kinases are typically localized to the inner side of the plasma membrane, where they act as signal transducers, facilitating communication between extracellular signals and intracellular responses. The phosphorylation of tyrosine residues on target proteins creates binding sites for downstream signaling molecules, thereby propagating the signal within the cell. Anti-phosphotyrosine antibody (2C8) specifically recognizes phosphotyrosine residues, making this monoclonal antibody an invaluable tool for researchers aiming to characterize and purify proteins involved in these critical signaling pathways, enhancing our understanding of cellular functions and disease mechanisms.

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Phosphotyrosine Antibody (2C8) References:

1. Structural and evolutionary division of phosphotyrosine binding (PTB) domains.  |  Uhlik, MT., et al. 2005. J Mol Biol. 345: 1-20. PMID: 15567406
2. Use of vanadate as protein-phosphotyrosine phosphatase inhibitor.  |  Gordon, JA. 1991. Methods Enzymol. 201: 477-82. PMID: 1943774
3. The language of SH2 domain interactions defines phosphotyrosine-mediated signal transduction.  |  Liu, BA., et al. 2012. FEBS Lett. 586: 2597-605. PMID: 22569091
4. A phosphotyrosine switch regulates organic cation transporters.  |  Sprowl, JA., et al. 2016. Nat Commun. 7: 10880. PMID: 26979622
5. Coupled membrane lipid miscibility and phosphotyrosine-driven protein condensation phase transitions.  |  Chung, JK., et al. 2021. Biophys J. 120: 1257-1265. PMID: 33080222
6. Phosphotyrosine couples peptide binding and SHP2 activation via a dynamic allosteric network.  |  Marasco, M., et al. 2021. Comput Struct Biotechnol J. 19: 2398-2415. PMID: 34025932
7. Late Stage Phosphotyrosine Mimetic Functionalization of Peptides Employing Metallaphotoredox Catalysis.  |  Chen, H., et al. 2021. Org Lett. 23: 4244-4249. PMID: 34029466
8. Optimization of Phosphotyrosine Peptides that Target the SH2 Domain of SOCS1 and Block Substrate Ubiquitination.  |  Chen, H., et al. 2022. ACS Chem Biol. 17: 449-462. PMID: 34989544
9. Genetic encoding of a nonhydrolyzable phosphotyrosine analog in mammalian cells.  |  He, X., et al. 2022. Chem Commun (Camb). 58: 5897-5900. PMID: 35474127
10. Dynamic Phosphotyrosine-Dependent Signaling Profiling in Living Cells by Two-Dimensional Proximity Proteomics.  |  Kong, Q., et al. 2022. J Proteome Res. 21: 2727-2735. PMID: 36280823

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Phosphotyrosine Antibody (2C8) has 7 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Phosphotyrosine Antibody (2C8) Specifications

Alternate Product Name: phosphotyrosine

Product Clone Name: 2C8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: N/A

Epitope Target: p-Tyr

Epitope Species: human

Product Reactivity/Detection: phosphotyrosine-containing proteins

Species Reactivity/Detection:

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Phosphotyrosine Antibody (2C8): N/A

The preferred Phosphotyrosine antibody is Phosphotyrosine Antibody (PY99): sc-7020.