p-Thr Antibody (14B3): sc-81526
- p-Thr Antibody (14B3) is a mouse monoclonal IgG1, cited in 3 publications, provided at 50 µg/0.5 ml
- raised against a synthetic threonine-phosphorylated peptide
- recommended for detection of phosphothreonine-containing proteins of mouse, rat, human and canine origin by WB and IP
- See p-Thr (H-2): sc-5267 for p-Thr antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Thr Antibody (14B3). This work is in progress.
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SEE ALSO...
p-Thr Antibody (14B3) is a mouse monoclonal IgG1 antibody that detects phosphothreonine in mouse, rat, human, and canine samples through applications such as western blotting (WB) and immunoprecipitation (IP). p-Thr monoclonal antibody (14B3) specifically recognizes phosphorylated threonine residues, which are critical for regulating various cellular processes, including signal transduction pathways. Phosphorylation of threonine plays a pivotal role in activating key mitogen-activated protein kinases (MAPKs) such as ERK and JNK, which mediate cellular responses to growth factors and stress signals. p-Thr (14B3) antibody enables researchers to investigate threonine phosphorylation dynamics and implications in cellular signaling, providing insights into mechanisms underlying various physiological and pathological conditions. p-Thr (14B3) monoclonal antibody serves as an invaluable tool for studying protein interaction networks and modifications that govern cellular behavior, advancing our understanding of cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p-Thr Antibody (14B3) References:
- Function of WW domains as phosphoserine- or phosphothreonine-binding modules. | Lu, PJ., et al. 1999. Science. 283: 1325-8. PMID: 10037602
- SpvC is a Salmonella effector with phosphothreonine lyase activity on host mitogen-activated protein kinases. | Mazurkiewicz, P., et al. 2008. Mol Microbiol. 67: 1371-83. PMID: 18284579
- Detection and assignment of phosphoserine and phosphothreonine residues by (13)C- (31)P spin-echo difference NMR spectroscopy. | McIntosh, LP., et al. 2009. J Biomol NMR. 43: 31-7. PMID: 19002654
- Structural and functional analysis of phosphothreonine-dependent FHA domain interactions. | Pennell, S., et al. 2010. Structure. 18: 1587-95. PMID: 21134638
- Computational investigation of the enzymatic mechanisms of phosphothreonine lyase. | Pei, Q., et al. 2011. Biophys Chem. 157: 16-23. PMID: 21558045
- OGlcNAcylation and phosphorylation have opposing structural effects in tau: phosphothreonine induces particular conformational order. | Brister, MA., et al. 2014. J Am Chem Soc. 136: 3803-16. PMID: 24559475
- The pellino e3 ubiquitin ligases recognize specific phosphothreonine motifs and have distinct substrate specificities. | Huoh, YS. and Ferguson, KM. 2014. Biochemistry. 53: 4946-55. PMID: 25027698
- Michael addition of dehydroalanine-containing MAPK peptides to catalytic lysine inhibits the activity of phosphothreonine lyase. | Zhang, Y., et al. 2015. FEBS Lett. 589: 3648-53. PMID: 26519561
- EYA1's Conformation Specificity in Dephosphorylating Phosphothreonine in Myc and Its Activity on Myc Stabilization in Breast Cancer. | Li, J., et al. 2017. Mol Cell Biol. 37: PMID: 27795300
- Threonine eliminylation by bacterial phosphothreonine lyases rapidly causes cross-linking of mitogen-activated protein kinase (MAPK) in live cells. | Meijer, BM., et al. 2017. J Biol Chem. 292: 7784-7794. PMID: 28325837
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Thr Antibody (14B3) | sc-81526 | 50 µg/0.5 ml | $322.00 |