p-SMC1 Antibody (5H24): sc-71789

p-SMC1 Antibody (5H24) is a mouse monoclonal IgG1 antibody that detects p-SMC1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). p-SMC1, a phosphorylated form of the structural maintenance of chromosomes protein 1, plays a crucial role in the regulation of sister chromatid cohesion and chromosome condensation during mitosis, which is essential for accurate cell division and genomic stability. The phosphorylation of SMC1 is particularly important as it is involved in the DNA damage response, allowing cells to maintain genomic integrity in the face of replication stress or DNA damage. This post-translational modification enhances SMC1′s interaction with other key regulators of the cell cycle, thereby facilitating the proper assembly of the cohesin complex. p-SMC1 is widely expressed across various human tissues, including the thymus, testis, and colon, highlighting SMC1′s fundamental role in cellular processes. anti-p-SMC1 antibody (5H24) effectively detects this critical protein, making p-SMC1 (5H24) antibody an invaluable tool for researchers studying cell cycle dynamics and the mechanisms underlying chromosomal stability.

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p-SMC1 Antibody (5H24) References:

1. Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway.  |  Kitagawa, R., et al. 2004. Genes Dev. 18: 1423-38. PMID: 15175241
2. Post-irradiation phosphorylation of structural maintenance chromosome 1 (SMC1) is independent of the Fanconi protein pathway.  |  Nahas, SA., et al. 2005. Int J Radiat Oncol Biol Phys. 61: 1167-72. PMID: 15752898
3. ATR regulates hexavalent chromium-induced S-phase checkpoint through phosphorylation of SMC1.  |  Wakeman, TP. and Xu, B. 2006. Mutat Res. 610: 14-20. PMID: 16876463
4. NBS1 prevents chromatid-type aberrations through ATM-dependent interactions with SMC1.  |  Antoccia, A., et al. 2008. Radiat Res. 170: 345-52. PMID: 18763866
5. Cohesin subunit SMC1 associates with mitotic microtubules at the spindle pole.  |  Wong, RW. and Blobel, G. 2008. Proc Natl Acad Sci U S A. 105: 15441-5. PMID: 18832153
6. Rapid flow cytometry-based structural maintenance of chromosomes 1 (SMC1) phosphorylation assay for identification of ataxia-telangiectasia homozygotes and heterozygotes.  |  Nahas, SA., et al. 2009. Clin Chem. 55: 463-72. PMID: 19147735
7. Blood-based detection of radiation exposure in humans based on novel phospho-Smc1 ELISA.  |  Ivey, RG., et al. 2011. Radiat Res. 175: 266-81. PMID: 21388270
8. Point mutation at the Nbs1 Threonine 278 site does not affect mouse development, but compromises the Chk2 and Smc1 phosphorylation after DNA damage.  |  Li, T. and Wang, ZQ. 2011. Mech Ageing Dev. 132: 382-8. PMID: 21664921
9. Human papillomaviruses activate and recruit SMC1 cohesin proteins for the differentiation-dependent life cycle through association with CTCF insulators.  |  Mehta, K., et al. 2015. PLoS Pathog. 11: e1004763. PMID: 25875106
10. An Escherichia coli Effector Protein EspF May Induce Host DNA Damage via Interaction With SMC1.  |  Fu, M., et al. 2021. Front Microbiol. 12: 682064. PMID: 34122393

The preferred SMC1 antibody is SMC1 Antibody (C2M): sc-56776.