p-Ser/Phosphoserine Antibody (4A3): sc-81516

p-Ser Antibody (4A3) is a mouse monoclonal IgM antibody that detects phosphorylated serine in mouse, rat, human, and canine samples through applications such as western blotting (WB) and immunoprecipitation (IP). p-Ser (4A3) antibody is available in a non-conjugated form, specifically designed to target a broad range of serine-phosphorylated proteins found in crude cell extracts, with a preference for positively charged amino acids adjacent to phosphoserine. Phosphorylation of serine residues is a critical post-translational modification that plays a vital role in regulating various cellular functions, including signal transduction, cell cycle progression, and apoptosis. The dynamic nature of serine phosphorylation allows cells to respond rapidly to external stimuli, making phosphoserine essential for maintaining cellular homeostasis and facilitating communication between different signaling pathways. Protein kinases, such as those from the Raf, Rsk, ROCK, PAK, Ak, and PKC families, are responsible for catalyzing this modification, and their activity can significantly influence the structure and function of target proteins. Using p-Ser (4A3) monoclonal antibody, researchers can effectively characterize proteins with phosphorylated serine residues and gain insights into the complex cellular pathways that involve serine phosphorylation, ultimately advancing our understanding of cellular signaling mechanisms.

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p-Ser/Phosphoserine Antibody (4A3) References:

1. Comparison of the binuclear metalloenzymes diphosphoglycerate-independent phosphoglycerate mutase and alkaline phosphatase: their mechanism of catalysis via a phosphoserine intermediate.  |  Jedrzejas, MJ. and Setlow, P. 2001. Chem Rev. 101: 607-18. PMID: 11712498
2. The use of in vitro peptide-library screens in the analysis of phosphoserine/threonine-binding domain structure and function.  |  Yaffe, MB. and Smerdon, SJ. 2004. Annu Rev Biophys Biomol Struct. 33: 225-44. PMID: 15139812
3. Phosphoserine Lyase Deoxyribozymes: DNA-Catalyzed Formation of Dehydroalanine Residues in Peptides.  |  Chandrasekar, J., et al. 2015. J Am Chem Soc. 137: 9575-8. PMID: 26200899
4. Targeting the phosphoserine phosphatase MtSerB2 for tuberculosis drug discovery, an hybrid knowledge based /fragment based approach.  |  Haufroid, M., et al. 2023. Eur J Med Chem. 245: 114935. PMID: 36403421
5. The protective effects of phosphoserine aminotransferase 1 (PSAT1) against hepatic ischemia-reperfusion injury.  |  Deng, Y. and Luo, H. 2023. J Cell Commun Signal. 17: 851-862. PMID: 36745318
6. A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis.  |  Pierson, E., et al. 2023. Commun Biol. 6: 1024. PMID: 37817000
7. Design of an apoptotic cell-mimetic wound dressing using phosphoserine-chitosan hydrogels.  |  Lee, G., et al. 2023. Biomater Sci. 11: 7897-7908. PMID: 37906511
8. Rationalizing the Influence of the Binding Affinity on the Activity of Phosphoserine Phosphatases.  |  Chiba, Y., et al. 2024. Angew Chem Int Ed Engl. 63: e202318635. PMID: 38408266
9. O-phosphoserine content of intermediate filament subunits.  |  Steinert, PM., et al. 1982. Biochemistry. 21: 177-83. PMID: 6174150
10. Influence of phosphoserine on the performance of rats on the rotarod.  |  Fanelli, O. 1976. Pharmacology. 14: 52-7. PMID: 959308

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Ser/Phosphoserine Antibody (4A3) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Ser/Phosphoserine Antibody (4A3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Ser/Phosphoserine Antibody (4A3) Specifications

Alternate Product Name: phosphorylated serine

Product Clone Name: 4A3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: N/A

Epitope Target: p-Ser

Epitope Species:

Product Reactivity/Detection: a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine

Species Reactivity/Detection: mouse, rat, human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgM kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for p-Ser/Phosphoserine Antibody (4A3): N/A

The preferred p-Ser/Phosphoserine antibody is p-Ser/Phosphoserine Antibody (16B4): sc-81514.