p-Ser/Phosphoserine Antibody (16B4): sc-81514

p-Ser Antibody (16B4) is a mouse monoclonal IgM kappa antibody that detects p-Ser protein of mouse, rat, human, and canine origin by western blotting (WB) and immunoprecipitation (IP). Anti-p-Ser antibody (16B4) is available as the non-conjugated form. p-Ser protein plays a crucial role in cellular signaling, as p-Ser serves as a key target for various protein kinases that catalyze the phosphorylation of serine residues. This post-translational modification is vital for regulating numerous cellular processes, including cell growth, differentiation, and apoptosis. Phosphorylation of serine can induce significant conformational changes in proteins, which may enhance or inhibit enzymatic activity and influence interactions with other proteins. Understanding serine phosphorylation dynamics is essential for elucidating complex signaling pathways and can provide insights into various diseases, including cancer and neurodegenerative disorders. Anti-p-Ser antibody (16B4) serves as an invaluable tool for researchers aiming to characterize proteins with phosphorylated serine residues and to explore intricate networks of cellular signaling that govern physiological and pathological states.

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p-Ser/Phosphoserine Antibody (16B4) References:

1. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
2. Phosphoserine Aminotransferase has Conserved Active Site from Microbes to Higher Eukaryotes with Minor Deviations.  |  Singh, RK., et al. 2021. Protein Pept Lett. 28: 996-1008. PMID: 33588715
3. Downregulation of phosphoserine phosphatase potentiates tumor immune environments to enhance immune checkpoint blockade therapy.  |  Peng, ZP., et al. 2023. J Immunother Cancer. 11: PMID: 36849198
4. Phosphoserine-loaded chitosan membranes promote bone regeneration by activating endogenous stem cells.  |  Ke, Y., et al. 2023. Front Bioeng Biotechnol. 11: 1096532. PMID: 37034248
5. Biosynthesis and Genetic Encoding of Non-hydrolyzable Phosphoserine into Recombinant Proteins in Escherichia coli.  |  Zhu, P., et al. 2023. Bio Protoc. 13: e4861. PMID: 37969748
6. Excess phosphoserine-129 α-synuclein induces synaptic vesicle trafficking and declustering defects at a vertebrate synapse.  |  Wallace, JN., et al. 2024. Mol Biol Cell. 35: ar10. PMID: 37991902
7. Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms.  |  Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
8. Constitutive mutant and putative regulatory serine phosphorylation site of mammalian MAP kinase kinase (MEK1).  |  Pagès, G., et al. 1994. EMBO J. 13: 3003-10. PMID: 8039496
9. Phosphorylation of the c-Fos transrepression domain by mitogen-activated protein kinase and 90-kDa ribosomal S6 kinase.  |  Chen, RH., et al. 1993. Proc Natl Acad Sci U S A. 90: 10952-6. PMID: 8248197
10. ROCK-I and ROCK-II, two isoforms of Rho-associated coiled-coil forming protein serine/threonine kinase in mice.  |  Nakagawa, O., et al. 1996. FEBS Lett. 392: 189-93. PMID: 8772201
11. Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway.  |  Brown, JL., et al. 1996. Curr Biol. 6: 598-605. PMID: 8805275
12. Novel phosphoserine phosphatase inhibitors.  |  Hawkinson, JE., et al. 1997. Eur J Pharmacol. 337: 315-24. PMID: 9430431

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Ser/Phosphoserine Antibody (16B4) has 95 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Ser/Phosphoserine Antibody (16B4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Ser/Phosphoserine Antibody (16B4) Specifications

Alternate Product Name: phosphorylated serine

Product Clone Name: 16B4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: N/A

Epitope Target: p-Ser

Epitope Species:

Product Reactivity/Detection: a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine

Species Reactivity/Detection: mouse, rat, human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgM kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 95 citations in various scientific publications

Available Conjugates/Forms for p-Ser/Phosphoserine Antibody (16B4): N/A