p-PKC beta II/ delta Antibody (E-7) recommended for detection of Ser 660 phosphorylated PKC βII of mouse, rat and human origin, correspondingly...by WB, IP, IF, IHC(P) and ELISA

p-PKC βII/δ Antibody (E-7): sc-365463

p-PKC βII/δ Antibody (E-7) is rated 4.8 out of 5 by 6.
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Datasheets

    • p-PKC βII/δ Antibody (E-7) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against an amino acid sequence containing phosphorylated Ser 660 of PKC βII of human origin
    • p-PKC beta II/ delta Antibody (E-7) is recommended for detection of Ser 660 phosphorylated PKC βII of mouse, rat and human origin, correspondingly Ser 662 phosphorylated PKC δ of mouse and rat origin, and correspondingly Ser 664 phosphorylated PKC δ of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-p-PKC beta II/ delta Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-365463 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-PKC βII/δ (E-7): sc-365463.

Dear Madam/Sir, Does sc-365463 antibody recognize also alpha-PKC isoform when phosphotylated at residue corresponding to Ser660 of PKC beta II?

Asked by: Matthew2
Thank you for your question. p-PKC βII/δ Antibody (E-7): sc-365463 recognizes Ser 660 phosphorylated PKC βII (mouse, rat, or human origin), Ser 662 phosphorylated PKC δ (mouse or rat origin), and Ser 664 phosphorylated PKC δ (human origin) by WB, IP, IF, IHC(P) and ELISA. These two antibodies are recommended for the detection of p-PKC α: p-PKC α Antibody (A-11) sc-377565 https://www.scbt.com/scbt/product/p-pkc-alpha-antibody-a-11 p-PKC α Antibody (pT638.35) sc-517540 https://www.scbt.com/scbt/product/p-pkc-alpha-antibody-pt638-35
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Does the antibody detect both phosphorylated PKC beta and delta?

Asked by: Dhanalakshmi
Yes, this antibody is recommended for detection of Ser 660 phosphorylated PKC βII of mouse, rat and human origin, correspondingly Ser 662 phosphorylated PKC δ of mouse and rat origin, and correspondingly Ser 664 phosphorylated PKC δ of human origin.
Answered by: Technical Support
Date published: 2018-12-21

What application is the blocking peptide sc-365463 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from gut The antibody worked very well at 1:500 dilution on THP-1 cells stimulated with PMA but the delta band is more or less absent.
Date published: 2019-03-05
Rated 5 out of 5 by from perfect antibody perfect results with specific band for target protein
Date published: 2017-12-04
Rated 5 out of 5 by from works great on western blot and IHC We have used this antibody on human skin tissue. It works great on both western blot and IHC.
Date published: 2017-09-20
Rated 5 out of 5 by from Very potent and specific antibody This monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is highly recommended for Western blotting. There was very strong detection of ~84 kDa protein that may be PKC-beta in human HeLa cells treated for 2 hours with anisomycin on Western blots, but no significant cross-reactivities on these blots with any off-target proteins.
Date published: 2016-12-04
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, positive in HeLa. -SCBT QC
Date published: 2015-01-09
Rated 5 out of 5 by from Expected results obtained by Western blot Expected results obtained by Western blot analysis of PKC Il/ phosphorylation in untreated and lambda protein phosphatase treated HeLa whole cell lysates. -SCBT QC
Date published: 2014-03-11
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