p-PACT Antibody (1A6-1H4): sc-73478
- p-PACT Antibody (1A6-1H4) is a mouse monoclonal IgG1 provided at 200 µg/ml
- raised against a synthetic peptide corresponding to amino acids Ser 18 to 25, including phosphorylated serine 18, of mouse PACT protein
- recommended for detection of Ser 18 phosphorylated PACT of mouse and rat origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-PACT Antibody (1A6-1H4). This work is in progress.
QUICK LINKS
SEE ALSO...
p-PACT Antibody (1A6-1H4) is a mouse monoclonal IgG1 antibody that detects PACT in both mouse and rat samples through applications such as western blotting (WB) and immunoprecipitation (IP). anti-PACT antibody (1A6-1H4) specifically recognizes the phosphorylated form of PACT, which plays a crucial role in the cellular response to stress by activating protein kinase PKR. This activation leads to the phosphorylation of eIF2α, a key regulator of protein synthesis, thereby inhibiting translation and promoting apoptosis under conditions such as serum starvation or oxidative stress. PACT′s function lies in modulating cell survival and death, making PACT a significant player in the cellular stress response. PACT′s interaction with double-stranded RNA (dsRNA) is noteworthy; however, eIF2α activation occurs only in the absence of dsRNA, indicating a complex regulatory mechanism. PACT undergoes phosphorylation at Serine 18, and the non-phosphorylated form is ineffective in activating PKR, underscoring the importance of post-translational modifications in PACT′s function. PACT′s ability to form heterodimers and interact with eIF2α through its DRBM domain further illustrates PACT′s intricate role in cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p-PACT Antibody (1A6-1H4) References:
- Viral stress-inducible genes. | Sen, GC. and Peters, GA. 2007. Adv Virus Res. 70: 233-63. PMID: 17765707
- Regulation of PACT-Mediated Protein Kinase Activation by the OV20.0 Protein of Orf Virus. | Tseng, YY., et al. 2015. J Virol. 89: 11619-29. PMID: 26355092
- Suppression of Type I Interferon Production by Human T-Cell Leukemia Virus Type 1 Oncoprotein Tax through Inhibition of IRF3 Phosphorylation. | Yuen, CK., et al. 2016. J Virol. 90: 3902-3912. PMID: 26819312
- Thiamine and dystonia 16. | Costantini, A., et al. 2016. BMJ Case Rep. 2016: PMID: 27448549
- A truncated PACT protein resulting from a frameshift mutation reported in movement disorder DYT16 triggers caspase activation and apoptosis. | Burnett, SB., et al. 2019. J Cell Biochem. 120: 19004-19018. PMID: 31246344
- Dystonia 16 (DYT16) mutations in PACT cause dysregulated PKR activation and eIF2α signaling leading to a compromised stress response. | Burnett, SB., et al. 2020. Neurobiol Dis. 146: 105135. PMID: 33049316
- SARS-CoV-2 NSP5 and N protein counteract the RIG-I signaling pathway by suppressing the formation of stress granules. | Zheng, Y., et al. 2022. Signal Transduct Target Ther. 7: 22. PMID: 35075101
- PACT inhibits the replication of SARS-CoV-2 through the blockage of GSK-3β-N-nsp3 cascade. | Lin, YY., et al. 2023. J Med Virol. 95: e28832. PMID: 37264691
- A mechanism that transduces lysosomal damage signals to stress granule formation for cell survival. | Duran, J., et al. 2024. bioRxiv.. PMID: 38617306
- Calcium signaling from damaged lysosomes induces cytoprotective stress granules. | Duran, J., et al. 2024. EMBO J. 43: 6410-6443. PMID: 39533058
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-PACT Antibody (1A6-1H4) | sc-73478 | 200 µg/ml | $316.00 |