p-p53 Antibody (D-9): sc-377567

p-p53 Antibody (D-9) is a mouse monoclonal IgM kappa light chain antibody that detects Thr 155 phosphorylated p53 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-p-p53 antibody (D-9) is available as the non-conjugated form. p53 plays a critical role as a tumor suppressor by regulating the cell cycle and promoting apoptosis in response to cellular stress, thereby preventing the proliferation of potentially cancerous cells. p53 function is vital for maintaining genomic stability, as p53 activates a network of genes involved in growth arrest and apoptosis, which are essential for cellular homeostasis. p53 is primarily located in the nucleus, where p53 binds to DNA to exert regulatory effects; however, p53 can be sequestered in the cytoplasm by the E3 ubiquitin ligase MDM2, which is a negative regulator that targets p53 for proteasomal degradation. This dynamic localization allows for a rapid response to cellular stress signals, ensuring that p53 is available to activate target genes when needed. The phosphorylation of p53 at Thr 155, mediated by the COP9 signalosome, is a key post-translational modification that influences stability and activity, highlighting the intricate regulatory mechanisms that control p53 function and implications in cancer biology. p-p53 monoclonal antibody (D-9) serves as an essential tool for investigating the regulatory pathways and functional dynamics of p53 in cellular stress responses and cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-p53 Antibody (D-9) References:

1. p53 website and analysis of p53 gene mutations in human cancer: forging a link between epidemiology and carcinogenesis.  |  Soussi, T., et al. 2000. Hum Mutat. 15: 105-13. PMID: 10612830
2. Regulation of p53 stability.  |  Ashcroft, M. and Vousden, KH. 1999. Oncogene. 18: 7637-43. PMID: 10618703
3. COP9 signalosome-specific phosphorylation targets p53 to degradation by the ubiquitin system.  |  Bech-Otschir, D., et al. 2001. EMBO J. 20: 1630-9. PMID: 11285227
4. The role of tetramerization in p53 function.  |  Chène, P. 2001. Oncogene. 20: 2611-7. PMID: 11420672
5. Distinct pattern of p53 phosphorylation in human tumors.  |  Minamoto, T., et al. 2001. Oncogene. 20: 3341-7. PMID: 11423984
6. Regulation of the specific DNA binding function of p53.  |  Hupp, TR., et al. 1992. Cell. 71: 875-86. PMID: 1423635
7. Arecoline-induced phosphorylated p53 and p21(WAF1) protein expression is dependent on ATM/ATR and phosphatidylinositol-3-kinase in clone-9 cells.  |  Chou, WW., et al. 2009. J Cell Biochem. 107: 408-17. PMID: 19343784
8. Dimethylfumarate inhibits MIF-induced proliferation of keratinocytes by inhibiting MSK1 and RSK1 activation and by inducing nuclear p-c-Jun (S63) and p-p53 (S15) expression.  |  Gesser, B., et al. 2011. Inflamm Res. 60: 643-53. PMID: 21340650
9. FBXW7-mutated colorectal cancer cells exhibit aberrant expression of phosphorylated-p53 at Serine-15.  |  Li, N., et al. 2015. Oncotarget. 6: 9240-56. PMID: 25860929
10. p53, the cellular gatekeeper for growth and division.  |  Levine, AJ. 1997. Cell. 88: 323-31. PMID: 9039259

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-p53 Antibody (D-9) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-p-p53 Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-p53 Antibody (D-9) Specifications

Alternate Product Name: TP53

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: Thr 155

Epitope Target: p53

Epitope Species: human

Product Reactivity/Detection: Thr 155 phosphorylated p53

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for p-p53 Antibody (D-9): P: Blocking Peptide

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.