p-Nitrophenyl Formate (CAS 1865-01-6)
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p-Nitrophenyl formate is an ester of formic acid and p-nitrophenol, serving as a reagent in synthetic and analytical chemistry due to its unique reactivity profile. Its structure, characterized by the nitro group attached to the phenyl ring, significantly influences its chemical behavior, enhancing its susceptibility to nucleophilic attack. This property is pivotal in studies involving esterase and protease enzyme activities, where p-nitrophenyl formate acts as a chromogenic substrate. Upon enzymatic cleavage, it liberates p-nitrophenol, a compound that can be quantitatively measured through its absorbance, thus enabling researchers to meticulously investigate enzyme kinetics and mechanisms. Moreover, its application extends into the synthesis of complex organic molecules, where it is utilized in transesterification reactions as a leaving group, showcasing its versatility and importance in facilitating the exploration and elucidation of biochemical pathways and synthetic strategies.
p-Nitrophenyl Formate (CAS 1865-01-6) References
- Purification and properties of a novel extra-cellular thermotolerant metallolipase of Bacillus coagulans MTCC-6375 isolate. | Kanwar, SS., et al. 2006. Protein Expr Purif. 46: 421-8. PMID: 16290008
- Purification of a moderate thermotolerant Bacillus coagulans BTS1 lipase and its properties in a hydro-gel system. | Kanwar, SS., et al. 2006. Acta Microbiol Immunol Hung. 53: 77-87. PMID: 16696551
- Synthesis of linear polyether polyol derivatives as new materials for bioconjugation. | Li, Z. and Chau, Y. 2009. Bioconjug Chem. 20: 780-9. PMID: 19275208
- Purification and characterization of a low molecular mass alkaliphilic lipase of Bacillus cereus MTCC 8372. | Verma, ML. and Kanwar, SS. 2010. Acta Microbiol Immunol Hung. 57: 191-207. PMID: 20870591
- Isolation of lipase producing thermophilic bacteria: optimization of production and reaction conditions for lipase from Geobacillus sp. | Mehta, A., et al. 2012. Acta Microbiol Immunol Hung. 59: 435-50. PMID: 23195552
- Purification and physicochemical properties of lipase from thermophilic Bacillus aerius. | Saun, NK., et al. 2014. J Oleo Sci. 63: 1261-8. PMID: 25391687
- Production and characterization of biodiesel using nonedible castor oil by immobilized lipase from Bacillus aerius. | Narwal, SK., et al. 2015. Biomed Res Int. 2015: 281934. PMID: 25874205
- Ancistectorine D, a naphthylisoquinoline alkaloid with antiprotozoal and antileukemic activities, and further 5,8'- and 7,1'-linked metabolites from the Chinese liana Ancistrocladus tectorius. | Bringmann, G., et al. 2016. Fitoterapia. 115: 1-8. PMID: 27646602
- On-resin N-formylation of peptides: a head-to-head comparison of reagents in solid-phase synthesis of ligands for formyl peptide receptors. | Christensen, SB., et al. 2017. J Pept Sci. 23: 410-415. PMID: 28421689
- Purification of lipase from Aspergillus fumigatus using Octyl Sepharose column chromatography and its characterization. | Mehta, A., et al. 2018. J Basic Microbiol. 58: 857-866. PMID: 30039877
- The role of formylmethanofuran: tetrahydromethanopterin formyltransferase in methanogenesis from carbon dioxide. | Donnelly, MI. and Wolfe, RS. 1986. J Biol Chem. 261: 16653-9. PMID: 3097011
- Characterization and synthetic biology of lipase from Bacillus amyloliquefaciens strain. | Khan, MT., et al. 2020. Arch Microbiol. 202: 1497-1506. PMID: 32219482
- The formation of N-formyl-methionyl-sRNA. | Marcker, K. 1965. J Mol Biol. 14: 63-70. PMID: 5327658
- Synthesis and characterization of (1-13C) Phe9 gramicidin A. Effects of side chain variations. | Prasad, KU., et al. 1983. Int J Pept Protein Res. 22: 341-7. PMID: 6195120
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Nitrophenyl Formate, 25 g | sc-212510 | 25 g | $286.00 |